Quantitative assessment of organ distribution ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Quantitative assessment of organ distribution of dietary protein-bound C-13-labeled N-epsilon-carboxymethyllysine after a chronic oral exposure in mice
Auteur(s) :
Tessier, Frederic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Niquet-Leridon, Celine [Auteur]
Institut Polytechnique LaSalle Beauvais
Jacolot, Philippe [Auteur]
Institut Polytechnique LaSalle Beauvais
Jouquand, Celine [Auteur]
Institut Polytechnique LaSalle Beauvais
Genin, Michaël [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Schmidt Ann, Marie [Auteur]
New York University Langone Medical Center [NYU Langone Medical Center]
Grossin, Nicolas [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Boulanger, Eric [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]
Niquet-Leridon, Celine [Auteur]
Institut Polytechnique LaSalle Beauvais
Jacolot, Philippe [Auteur]
Institut Polytechnique LaSalle Beauvais
Jouquand, Celine [Auteur]
Institut Polytechnique LaSalle Beauvais
Genin, Michaël [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Schmidt Ann, Marie [Auteur]
New York University Langone Medical Center [NYU Langone Medical Center]
Grossin, Nicolas [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Boulanger, Eric [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Titre de la revue :
Molecular nutrition & food research
Nom court de la revue :
Mol. Nutr. Food Res.
Numéro :
60
Pagination :
2446-2456
Éditeur :
Wiley Online Library
Date de publication :
2016-11-01
ISSN :
1613-4125
Mot(s)-clé(s) :
Carboxymethyllysine
RAGE
Mass spectrometry
Glycation
Food
Biodistribution
RAGE
Mass spectrometry
Glycation
Food
Biodistribution
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Scope
Nɛ-Carboxymethyl-lysine (CML) is a prominent advanced glycation end-product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation ...
Lire la suite >Scope Nɛ-Carboxymethyl-lysine (CML) is a prominent advanced glycation end-product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found. Methods and results A new protocol which discriminates dCML from native CML (nCML) has been developed. Three CML isotopes with different mass-to-charge ratios were used: nCML Nε-carboxymethyl-L-lysine, dCML Nε-[13C]carboxy[13C]methyl-L-lysine and internal standard Nε-carboxymethyl-L-[4,4,5,5-2H4]lysine. Wild-type (n = 7) and RAGE−/− (n = 8) mice were fed for 30 days with either a control, or a BSA-bound dCML-enriched diet. Organs were analyzed for nCML and dCML using liquid chromatography–tandem mass spectrometry. Mice exposed to dCML showed an accumulation in all tissues tested except fat. The rate of deposition was high (81–320 μgdCML/g dry matter) in kidneys, intestine, and lungs and low (<5 μg/g) in heart, muscle, and liver. This accumulation was not RAGE dependent. Conclusion The kidney is not the only organ affected by the accumulation of dCML. Its high accumulation in other tissues and organs may also, however, have important physiological consequences.Lire moins >
Lire la suite >Scope Nɛ-Carboxymethyl-lysine (CML) is a prominent advanced glycation end-product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found. Methods and results A new protocol which discriminates dCML from native CML (nCML) has been developed. Three CML isotopes with different mass-to-charge ratios were used: nCML Nε-carboxymethyl-L-lysine, dCML Nε-[13C]carboxy[13C]methyl-L-lysine and internal standard Nε-carboxymethyl-L-[4,4,5,5-2H4]lysine. Wild-type (n = 7) and RAGE−/− (n = 8) mice were fed for 30 days with either a control, or a BSA-bound dCML-enriched diet. Organs were analyzed for nCML and dCML using liquid chromatography–tandem mass spectrometry. Mice exposed to dCML showed an accumulation in all tissues tested except fat. The rate of deposition was high (81–320 μgdCML/g dry matter) in kidneys, intestine, and lungs and low (<5 μg/g) in heart, muscle, and liver. This accumulation was not RAGE dependent. Conclusion The kidney is not the only organ affected by the accumulation of dCML. Its high accumulation in other tissues and organs may also, however, have important physiological consequences.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Inserm
Université de Lille
CHU Lille
Université de Lille
CHU Lille
Collections :
Équipe(s) de recherche :
Glycation from inflammation to aging
Date de dépôt :
2019-03-01T15:16:23Z
2022-09-21T08:14:09Z
2022-09-21T08:14:09Z