Titre :

Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization

Auteur(s) :

Schwarzer, M. [Auteur]
Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] [MBU / CAS]
Repa, A. [Auteur]
Medizinische Universität Wien = Medical University of Vienna
Daniel, C. [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Schabussova, I. [Auteur]
Medizinische Universität Wien = Medical University of Vienna
Hrncir, T. [Auteur]
Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] [MBU / CAS]
Pot, B. [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Stepankova, R. [Auteur]
Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] [MBU / CAS]
Hudcovic, T. [Auteur]
Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] [MBU / CAS]
Pollak, A. [Auteur]
Medizinische Universität Wien = Medical University of Vienna
Tlaskalova-Hogenova, H. [Auteur]
Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] [MBU / CAS]
Wiedermann, U. [Auteur correspondant]
Medizinische Universität Wien = Medical University of Vienna
Kozakova, H. [Auteur]
Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] [MBU / CAS]

Titre de la revue :

Allergy

Pagination :

368-375

Éditeur :

Wiley

Date de publication :

2011-03

ISSN :

0105-4538

Mot(s)-clé(s) en anglais :

allergy
germ-free mice
mucosal immunity
primary prevention
recombinant lactic acid bacteria

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Background: The use of recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery of recombinant allergens is an attractive concept for antigen-defined allergy prevention/treatment. Interventions with LAB are ...
Lire la suite >Background: The use of recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery of recombinant allergens is an attractive concept for antigen-defined allergy prevention/treatment. Interventions with LAB are of increasing interest early in life when immune programming is initiated. Here, we investigated the effect of neonatal colonization with a recombinant LAB producing the major birch pollen allergen Bet v 1 in a murine model of type I allergy.Methods: We constructed a recombinant Lactobacillus (L.) plantarum NCIMB8826 strain constitutively producing Bet v 1 to be used for natural mother-to-offspring mono-colonization of germ-free BALB/c mice. Allergen-specific immunomodulatory effects of the colonization on humoral and cellular immune responses were investigated prior and after sensitization to Bet v 1.Results: Mono-colonization with the Bet v 1 producing L. plantarum induced a Th1-biased immune response at the cellular level, evident in IFN-γ production of splenocytes upon stimulation with Bet v 1. After sensitization with Bet v 1 these mice displayed suppressed IL-4 and IL-5 production in spleen and mesenteric lymph node cell cultures as well as decreased allergen-specific antibody responses (IgG1, IgG2a, and IgE) in sera. This suppression was associated with a significant up-regulation of the regulatory marker Foxp3 at the mRNA level in the spleen cells.Conclusion: Intervention at birth with a live recombinant L. plantarum producing a clinically relevant allergen reduces experimental allergy and might therefore become an effective strategy for early intervention against the onset of allergic diseases.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL