Title :

Subcellular imaging during single cell mechanical characterization

Author(s) :

Pekin, Deniz [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Perret, Gregoire [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Rezard, Quentin [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Gerbedoen, Jean Claude [Auteur]
Meignan, Samuel [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Centre National de la Recherche Scientifique [CNRS]
Collard, Dominique [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Centre National de la Recherche Scientifique [CNRS]
Lagadec, Chann [Auteur]
Signalisation des facteurs de croissance dans le cancer du sein. Protéomique fonctionnelle
Université de Lille, Sciences et Technologies
Tarhan, Mehmet-Cagatay [Auteur correspondant]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]

Conference title :

33rd IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

City :

Vancouver

Country :

Canada

Start date of the conference :

2020-01-18

Book title :

33rd IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

Publisher :

IEEE

Publication date :

2020

English keyword(s) :

Subcellular imaging
single cell analysis
mechanical characterization
MEMS tweezers
confocal microscopy
cancer

HAL domain(s) :

Sciences de l'ingénieur [physics]

English abstract : [en]

We report a method for combining confocal microscopy with single-cell mechanical characterization. This method allows investigating the effect of subcellular deformation (e.g. membranes, cytoplasm, cytoskeleton and organelles ...
Show more >We report a method for combining confocal microscopy with single-cell mechanical characterization. This method allows investigating the effect of subcellular deformation (e.g. membranes, cytoplasm, cytoskeleton and organelles as endoplasmic reticulum, mitochondria, and nucleus) on cell mechanical properties (e.g. stiffness and viscosity). Such a method is essential to choose biologically relevant mechanical properties of malignant cells as diagnostic cancer biomarkers. Using MEMS tweezers, we captured live single cancer cells and performed subcellular imaging during compression assays for mechanical measurements to obtain the deformation-dependent cell properties.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Comment :

oral

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL