TLR5 signalling is hyper-responsive in ...
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
TLR5 signalling is hyper-responsive in porcine cystic fibrosis airways epithelium
Author(s) :
Fleurot, Isabelle [Auteur]
Infectiologie et Santé Publique [ISP]
López-Gálvez, Raquel [Auteur]
Infectiologie et Santé Publique [ISP]
Barbry, Pascal [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Guillon, Antoine [Auteur]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
Si-Tahar, Mustapha [Auteur]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
Bähr, Andrea [Auteur]
Klymiuk, Nikolai [Auteur]
SIRARD, Jean-Claude [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Caballero, Ignacio [Auteur correspondant]
Infectiologie et Santé Publique [ISP]
Infectiologie et Santé Publique [ISP]
López-Gálvez, Raquel [Auteur]
Infectiologie et Santé Publique [ISP]
Barbry, Pascal [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Guillon, Antoine [Auteur]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
Si-Tahar, Mustapha [Auteur]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
Bähr, Andrea [Auteur]
Klymiuk, Nikolai [Auteur]
SIRARD, Jean-Claude [Auteur]

Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Caballero, Ignacio [Auteur correspondant]
Infectiologie et Santé Publique [ISP]
Journal title :
Journal of Cystic Fibrosis
Pages :
5 p.
Publisher :
Elsevier
Publication date :
2021-08
ISSN :
1569-1993
HAL domain(s) :
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Sciences du Vivant [q-bio]/Immunologie/Immunité innée
Sciences du Vivant [q-bio]/Immunologie/Immunité innée
English abstract : [en]
Excessive lung inflammation and airway epithelium damage are hallmarks of cystic fibrosis (CF) disease. It is unclear whether lung inflammation is related to an intrinsic defect in the immune response or to chronic infection. ...
Show more >Excessive lung inflammation and airway epithelium damage are hallmarks of cystic fibrosis (CF) disease. It is unclear whether lung inflammation is related to an intrinsic defect in the immune response or to chronic infection. We aimed to determine whether TLR5-mediated response is defective in the CF airway epithelium. We used a newborn CF pig model to study intrinsic alterations in CF airway epithelium innate immune response. Airway epithelial cells (AECs) were stimulated with flagellin or lipopolysaccharide to determine responses specific for TLR5 and TLR4, respectively. We observed a significant increase in cytokine secretion when CF AECs were stimulated with flagellin compared to wild type (WT) AECs. These results were recapitulated when AECs were treated with an inhibitor of CFTR channel activity. We show that TLR5-signalling is altered in CF lung epithelium at birth. Modulation of TLR5 signalling could contribute to better control the excessive inflammatory response observed in CF lungs.Show less >
Show more >Excessive lung inflammation and airway epithelium damage are hallmarks of cystic fibrosis (CF) disease. It is unclear whether lung inflammation is related to an intrinsic defect in the immune response or to chronic infection. We aimed to determine whether TLR5-mediated response is defective in the CF airway epithelium. We used a newborn CF pig model to study intrinsic alterations in CF airway epithelium innate immune response. Airway epithelial cells (AECs) were stimulated with flagellin or lipopolysaccharide to determine responses specific for TLR5 and TLR4, respectively. We observed a significant increase in cytokine secretion when CF AECs were stimulated with flagellin compared to wild type (WT) AECs. These results were recapitulated when AECs were treated with an inhibitor of CFTR channel activity. We show that TLR5-signalling is altered in CF lung epithelium at birth. Modulation of TLR5 signalling could contribute to better control the excessive inflammatory response observed in CF lungs.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
ANR Project :
Source :
Submission date :
2021-09-01T01:31:12Z
Files
- https://doi.org/10.1016/j.jcf.2021.08.002
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- https://doi.org/10.1016/j.jcf.2021.08.002
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