Titre :

Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis

Auteur(s) :

Maes, Emmanuel [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Sadovskaya, Irina [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Lévêque, Mathilde [Auteur]
Elass, Elisabeth [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Payré, Bruno [Auteur]
Grard, Thierry [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Théodorou, Vassilia [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Mercier-Bonin, Muriel [Auteur]

Titre de la revue :

Glycoconjugate journal

Nom court de la revue :

Glycoconj J

Numéro :

36

Pagination :

39-55

Date de publication :

2019-02

ISSN :

1573-4986

Mot(s)-clé(s) en anglais :

Cell Wall
Cytokines
HEK293 Cells
Hexosamines
Humans
Lactobacillus
Polysaccharides, Bacterial
Probiotics
Signal Transduction
Toll-Like Receptors
Cell wall polysaccharide
Immunomodulation
Intestinal barrier
Lactobacillus farciminis
Probiotic
Structure

Discipline(s) HAL :

Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified ...
Lire la suite >Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of: [→6αGlcpNAc1 → 4βManpNAc1 → 4βGlcpNAc1→] highly substituted with single residues of αGlcp, αGalp and αGlcpNAc. Subsequently, the lack of pro- or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-α/IFN-γ cytokine-mediated epithelium impairment.Lire moins >

Langue :

Anglais

Comité de lecture :

Non

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Glycobiologie structurale des interactions hôtes-pathogènes
Chemical Glycobiology

Plateforme(s) de recherche :

Plateforme d’Analyse des Glycoconjugués (PAGéS)

Date de dépôt :

2021-10-18T12:56:20Z
2021-10-19T15:36:10Z