Title :

Scavenger Receptors in Human Airway Epithelial Cells: Role in Response to Double-Stranded RNA

Author(s) :

Dieudonné, Audrey [Auteur]
Centre hospitalier [Valenciennes, Nord]
Médecine cellulaire et moléculaire [MCM]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Torres, David [Auteur]
Médecine cellulaire et moléculaire [MCM]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Blanchard, Simon [Auteur]
Centre Hospitalier Universitaire d'Angers [CHU Angers]
Centre de Recherche en Cancérologie Nantes-Angers [CRCNA]
Taront, Solenne [Auteur]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Médecine cellulaire et moléculaire [MCM]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Jeannin, Pascale [Auteur]
Centre Hospitalier Universitaire d'Angers [CHU Angers]
Centre de Recherche en Cancérologie Nantes-Angers [CRCNA]
Delneste, Yves [Auteur]
Centre Hospitalier Universitaire d'Angers [CHU Angers]
Centre de Recherche en Cancérologie Nantes-Angers [CRCNA]
Pichavant, Muriel [Auteur]
Médecine cellulaire et moléculaire [MCM]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Trottein, Francois [Auteur]
Médecine cellulaire et moléculaire [MCM]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Gosset, Philippe [Auteur correspondant]
Médecine cellulaire et moléculaire [MCM]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Journal title :

PLoS ONE

Pages :

e41952

Publisher :

Public Library of Science

Publication date :

2012-08-07

ISSN :

1932-6203

HAL domain(s) :

Sciences du Vivant [q-bio]/Immunologie

English abstract : [en]

Scavenger receptors and Toll-like receptors (TLRs) cooperate in response to danger signals to adjust the host immune response. The TLR3 agonist double stranded (ds)RNA is an efficient activator of innate signalling in ...
Show more >Scavenger receptors and Toll-like receptors (TLRs) cooperate in response to danger signals to adjust the host immune response. The TLR3 agonist double stranded (ds)RNA is an efficient activator of innate signalling in bronchial epithelial cells. In this study, we aimed at defining the role played by scavenger receptors expressed by bronchial epithelial cells in the control of the innate response to dsRNA both in vitro and in vivo. Expression of several scavenger receptor involved in pathogen recognition was first evaluated in human bronchial epithelial cells in steady-state and inflammatory conditions. Their implication in the uptake of dsRNA and the subsequent cell activation was evaluated in vitro by competition with ligand of scavenger receptors including maleylated ovalbumin and by RNA silencing. The capacity of maleylated ovalbumin to modulate lung inflammation induced by dsRNA was also investigated in mice. Exposure to tumor necrosis factor-a increased expression of the scavenger receptors LOX-1 and CXCL16 and the capacity to internalize maleylated ovalbumin, whereas activation by TLR ligands did not. In contrast, the expression of SR-B1 was not modulated in these conditions. Interestingly, supplementation with maleylated ovalbumin limited dsRNA uptake and inhibited subsequent activation of bronchial epithelial cells. RNA silencing of LOX-1 and SR-B1 strongly blocked the dsRNA-induced cytokine production. Finally, administration of maleylated ovalbumin in mice inhibited the dsRNA-induced infiltration and activation of inflammatory cells in bronchoalveolar spaces and lung draining lymph nodes. Together, our data characterize the function of SR-B1 and LOX-1 in bronchial epithelial cells and their implication in dsRNA-induced responses, a finding that might be relevant during respiratory viral infections.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL