Comparative Analysis of Hepatitis C Virus ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Title :
Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants
Author(s) :
Riva, Laura [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Spriet, Corentin [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Barois, Nicolas [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Popescu, Costin-Ioan [Auteur]
Institute of Biochemistry [Bucharest]
Dubuisson, Jean [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Rouillé, Yves [Auteur correspondant]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Spriet, Corentin [Auteur]

Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Barois, Nicolas [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Popescu, Costin-Ioan [Auteur]
Institute of Biochemistry [Bucharest]
Dubuisson, Jean [Auteur]

Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Rouillé, Yves [Auteur correspondant]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Journal title :
Pathogens
Pages :
172
Publisher :
MDPI
Publication date :
2021-02-04
ISSN :
2076-0817
English keyword(s) :
hepatitis C virus
NS5A dynamics
NS4B
core
lipid droplets
assembly
NS5A dynamics
NS4B
core
lipid droplets
assembly
HAL domain(s) :
Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
English abstract : [en]
The hepatitis C virus (HCV) life cycle is a tightly regulated process, during which structural and non-structural proteins cooperate. However, the interplay between HCV proteins during genomic RNA replication and progeny ...
Show more >The hepatitis C virus (HCV) life cycle is a tightly regulated process, during which structural and non-structural proteins cooperate. However, the interplay between HCV proteins during genomic RNA replication and progeny virion assembly is not completely understood. Here, we studied the dynamics and intracellular localization of non-structural 5A protein (NS5A), which is a protein involved both in genome replication and encapsidation. An NS5A-eGFP (enhanced green fluorescent protein) tagged version of the strain JFH-1-derived wild-type HCV was compared to the corresponding assembly-deficient viruses Δcore, NS5A basic cluster 352–533 mutant (BCM), and serine cluster 451 + 454 + 457 mutant (SC). These analyses highlighted an increase of NS5A motility when the viral protein core was lacking. Although to a lesser extent, NS5A motility was also increased in the BCM virus, which is characterized by a lack of interaction of NS5A with the viral RNA, impairing HCV genome encapsidation. This observation suggests that the more static NS5A population is mainly involved in viral assembly rather than in RNA replication. Finally, NS4B exhibited a reduced co-localization with NS5A and lipid droplets for both Δcore and SC mutants, which is characterized by the absence of interaction of NS5A with core. This observation strongly suggests that NS5A is involved in targeting NS4B to lipid droplets (LDs). In summary, this work contributes to a better understanding of the interplay between HCV proteins during the viral life cycle.Show less >
Show more >The hepatitis C virus (HCV) life cycle is a tightly regulated process, during which structural and non-structural proteins cooperate. However, the interplay between HCV proteins during genomic RNA replication and progeny virion assembly is not completely understood. Here, we studied the dynamics and intracellular localization of non-structural 5A protein (NS5A), which is a protein involved both in genome replication and encapsidation. An NS5A-eGFP (enhanced green fluorescent protein) tagged version of the strain JFH-1-derived wild-type HCV was compared to the corresponding assembly-deficient viruses Δcore, NS5A basic cluster 352–533 mutant (BCM), and serine cluster 451 + 454 + 457 mutant (SC). These analyses highlighted an increase of NS5A motility when the viral protein core was lacking. Although to a lesser extent, NS5A motility was also increased in the BCM virus, which is characterized by a lack of interaction of NS5A with the viral RNA, impairing HCV genome encapsidation. This observation suggests that the more static NS5A population is mainly involved in viral assembly rather than in RNA replication. Finally, NS4B exhibited a reduced co-localization with NS5A and lipid droplets for both Δcore and SC mutants, which is characterized by the absence of interaction of NS5A with core. This observation strongly suggests that NS5A is involved in targeting NS4B to lipid droplets (LDs). In summary, this work contributes to a better understanding of the interplay between HCV proteins during the viral life cycle.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
ANR Project :
Source :
Files
- https://hal-cnrs.archives-ouvertes.fr/hal-03432843/document
- Open access
- Access the document
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919264/pdf
- Open access
- Access the document
- https://hal-cnrs.archives-ouvertes.fr/hal-03432843/document
- Open access
- Access the document
- https://hal-cnrs.archives-ouvertes.fr/hal-03432843/document
- Open access
- Access the document
- document
- Open access
- Access the document
- Pathogens%202021%20RivaComparative%20Analysis%20of%20Hepatitis%20C%20Virus%20NS5A%20Dynamics%20and%20Localization%20in%20Assembly-Deficient%20Mutants.pdf
- Open access
- Access the document
- Open access
- Access the document
- document
- Open access
- Access the document
- Pathogens%202021%20RivaComparative%20Analysis%20of%20Hepatitis%20C%20Virus%20NS5A%20Dynamics%20and%20Localization%20in%20Assembly-Deficient%20Mutants.pdf
- Open access
- Access the document