Title :

Nisin-based coatings for the prevention of biofilm formation: Surface characterization and antimicrobial assessments

Author(s) :

Hage, Mayssane [Auteur]

Unité Matériaux et Transformations - UMR 8207 [UMET]
Chihib, Nour-Eddine [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Abdallah, Marwan [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Khelissa, Simon [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Crocco, Beatrice [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Akoum, Hikmat [Auteur]

Bentiss, Fouad [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Université Chouaib Doukkali [UCD]
Jama, charafeddine [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207

Journal title :

Surfaces and Interfaces

Volume number :

27

Pages :

101564

Publisher :

Elsevier BV

Publication date :

2021-12

ISSN :

2468-0230

English keyword(s) :

Nisin
Stainless steel
Listeria monocytogenes
Biofilms
Surface
Antimicrobial properties

HAL domain(s) :

Chimie/Matériaux
Chimie/Polymères
Physique [physics]/Matière Condensée [cond-mat]/Science des matériaux [cond-mat.mtrl-sci]
Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
Sciences du Vivant [q-bio]/Ingénierie des aliments
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
Sciences de l'ingénieur [physics]/Génie des procédés

English abstract : [en]

Pathogenic bacterial biofilms invading surfaces in food and medical fields are a challenge to overcome. Despite all the strategies applied to fight their formation, the microbiological risk associated to bacterial biofilms ...
Show more >Pathogenic bacterial biofilms invading surfaces in food and medical fields are a challenge to overcome. Despite all the strategies applied to fight their formation, the microbiological risk associated to bacterial biofilms remains an important threat for at risk population and for food and healthcare sectors. The prevention of biofilm formation might be an effective approach to confront this problem. In this study, stainless steel surfaces were functionalized by nisin, a natural antimicrobial peptide. The mechanism of action of immobilized nisin against sensitive bacteria is not fully understood. Therefore, nisin was grafted onto the surface by either its carboxylic group or its amino group. The generated coating's chemical, topographical and antibacterial properties were studied to understand the nisin mode of action, when immobilized, and identify the section of the bacteriocin responsible for the antimicrobial activity. The antimicrobial activity of the elaborated coatings was tested against Listeria monocytogenes. Indeed, the surfaces coated with nisin linked by its amino group showed an efficient antibacterial activity while the surface with nisin linked by its carboxylic group showed less antimicrobial effect. The antimicrobial results showed almost 2 log reduction of colony forming units for efficient antibacterial coatings while the other showed no bacterial reduction. The surface properties analysis permitted to understand the chemical and topographical characteristics of treated surfaces including nisin conformation and quantification. A tight relation was concluded between the surface topography, the nisin conformation, and the antibacterial activity of the bacteriocin-coated surfaces.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Ingénierie des Systèmes Polymères
Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2021-12-14T17:00:57Z
2021-12-16T15:50:13Z