Title :

High Dimensional Single-Cell Analysis Reveals iNKT Cell Developmental Trajectories and Effector Fate Decision

Author(s) :

Baranek, Thomas [Auteur]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]
LeBrigand, Kevin [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
de Amat Herbozo, Carolina [Auteur]
University of Toronto
Gonzalez, Loïc [Auteur]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]
Bogard, Gemma [Auteur]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]
Dietrich, Céline [Auteur]
Institut Necker Enfants-Malades [INEM - UM 111 (UMR 8253 / U1151)]
Magnone, Virginie [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Boisseau, Chloé [Auteur]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]
Jouan, Youenn [Auteur]
CHU Trousseau [Tours]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]
Trottein, François [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Si-Tahar, Mustapha [Auteur]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]
Leite-de-Moraes, Maria [Auteur]
Institut Necker Enfants-Malades [INEM - UM 111 (UMR 8253 / U1151)]
Mallevaey, Thierry [Auteur]
University of Toronto
Paget, Christophe [Auteur]
Centre d’Etude des Pathologies Respiratoires [Tours] [CEPR - U 1100]

Journal title :

Cell Reports

Pages :

108116

Publisher :

Elsevier Inc

Publication date :

2020-09

ISSN :

2211-1247

English keyword(s) :

FHL2
developmental program
iNKT cells
innate T cells
single-cell RNA sequencing
thymic egress
thymus
transcriptome

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

CD1d-restricted invariant Natural Killer T (iNKT) cells represent a unique class of T lymphocytes endowed with potent regulatory and effector immune functions. Although these functions are acquired during thymic ontogeny, ...
Show more >CD1d-restricted invariant Natural Killer T (iNKT) cells represent a unique class of T lymphocytes endowed with potent regulatory and effector immune functions. Although these functions are acquired during thymic ontogeny, the sequence of events that gives rise to discrete effector subsets remains unclear. Using an unbiased single-cell transcriptomic analysis combined with functional assays, we reveal an unappreciated diversity among thymic iNKT cells, especially among iNKT1 cells. Mathematical modeling and biological methods unravel a developmental map whereby iNKT2 cells constitute a transient branching point toward the generation of iNKT1 and iNKT17 cells, which reconciles the two previously proposed models. In addition, we identify the transcription co-factor Four-and-a-half LIM domains protein 2 (FHL2) as a critical cell-intrinsic regulator of iNKT1 specification. Thus, these data illustrate the changing transcriptional network that guides iNKT cell effector fate.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

Submission date :

2022-01-22T02:31:30Z