Title :

SERS characterization of aggregated and isolated bacteria deposited on silver-based substrates

Author(s) :

Andrei, Cristina-Cassiana [Auteur]
Laboratoire de physique de la matière condensée [LPMC]
Moraillon, Anne [Auteur]
Laboratoire de physique de la matière condensée [LPMC]
Potara, Monica [Auteur]
Astilean, Simion [Auteur]
Babes-Bolyai University [Cluj-Napoca] [UBB]
Jakab, Endre [Auteur]
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Rosselle, Léa [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Skandrani, Nadia [Auteur]
Boukherroub, Rabah [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Ozanam, François [Auteur]
Szunerits, Sabine [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Gouget-Laemmel, Anne Chantal [Auteur]

Journal title :

Analytical and Bioanalytical Chemistry

Abbreviated title :

Anal Bioanal Chem

Volume number :

413

Pages :

1417-1428

Publisher :

Springer Science and Business Media LLC

Publication date :

2021-01-03

ISSN :

1618-2642

English keyword(s) :

Surface-enhanced Raman scattering (SERS)
Silver nanostructures
Escherichia coli
Staphylococcus aureus
Aggregated and single bacteria
Principal component analysis (PCA)

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Surface-enhanced Raman scattering (SERS), based on the enhancement of the Raman signal of molecules positioned within a few nanometres from a structured metal surface, is ideally suited to provide bacterial-specific molecular ...
Show more >Surface-enhanced Raman scattering (SERS), based on the enhancement of the Raman signal of molecules positioned within a few nanometres from a structured metal surface, is ideally suited to provide bacterial-specific molecular fingerprints which can be used for analytical purposes. However, for some complex structures such as bacteria, the generation of reproducible SERS spectra is still a challenging task. Among the various factors influencing the SERS variability (such as the nature of SERS-active substrate, Raman parameters and bacterial specificity), we demonstrate in this study that the environment of Gram-positive and Gram-negative bacteria deposited on ultra-thin silver films also impacts the origin of the SERS spectra. In the case of densely packed bacteria, the obtained SERS signatures were either characteristic of the secretion of adenosine triphosphate for Staphylococcus aureus (S. aureus) or the cell wall and the pili/flagella for Escherichia coli (E. coli), allowing for an easy discrimination between the various strains. In the case of isolated bacteria, SERS mapping together with principal component analysis revealed some variabilities of the spectra as a function of the bacteria environment and the bactericidal effect of the silver. However, the variability does not preclude the SERS signatures of various E. coli strains to be discriminated.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Computational Molecular Systems Biology

Submission date :

2022-01-24T09:54:57Z
2022-01-26T09:49:36Z