Title :

Using human bronchial epithelial beas-2b cells: cytotoxicity, oxidative stress, pro-inflammatory response, genotoxicity, and cell cycle deregulation

Author(s) :

Abbas, Imane [Auteur]
Lebanese Atomic Energy Comission [CNRS-L] [LAEC-CNRS]
Badran, Ghidaa [Auteur]
Unité de Chimie Environnementale et Interactions sur le Vivant [UCEIV]
Verdin, Anthony [Auteur]
Unité de Chimie Environnementale et Interactions sur le Vivant [UCEIV]
Ledoux, Frederic [Auteur]
Unité de Chimie Environnementale et Interactions sur le Vivant [UCEIV]
Roumie, Mohamed [Auteur]
Lebanese Atomic Energy Comission [CNRS-L] [LAEC-CNRS]
Lo-Guidice, Jean-Marc [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Courcot, Dominique [Auteur]
Lebanese Atomic Energy Comission [CNRS-L] [LAEC-CNRS]
Garçon, Guillaume [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Journal title :

Environmental research

Abbreviated title :

Environ. Res.

Volume number :

171

Pages :

510-522

Publisher :

Elsevier

Publication date :

2019-01-31

ISSN :

1096-0953

Keyword(s) :

Genotoxicity
Organic extractable matter
BEAS-2B cells
Oxidative stress
Inflammation
Cell survival

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

A particular attention has been devoted to the type of toxicological responses induced by particulate matter (PM), since their knowledge is greatly complicated by the fact that it is a heterogeneous and often poorly described ...
Show more >A particular attention has been devoted to the type of toxicological responses induced by particulate matter (PM), since their knowledge is greatly complicated by the fact that it is a heterogeneous and often poorly described pollutant. However, despite intensive research effort, there is still a lack of knowledge about the specific chemical fraction of PM, which could be mainly responsible of its adverse health effects. We sought also to better investigate the toxicological effects of organic extractable matter (OEM) in normal human bronchial epithelial lung BEAS-2B cells. The wide variety of chemicals, including PAH and other related-chemicals, found in OEM, has been rather associated with early oxidative events, as supported by the early activation of the sensible NRF-2 signaling pathway. For the most harmful conditions, the activation of this signaling pathway could not totally counteract the ROS overproduction, thereby leading to critical oxidative damage to macromolecules (lipid peroxidation, oxidative DNA adducts). While NRF-2 is an anti-inflammatory, OEM exposure did not trigger any significant change in the secretion of inflammatory cytokines (i.e., TNFα, IL-1β, IL-6, IL-8, MCP-1, and IFNγ). According to the high concentrations of PAH and other related organic chemicals found in this OEM, CYP1A1 and 1B1 genes exhibited high transcription levels in BEAS-2B cells, thereby supporting both the activation of the critical AhR signaling pathway and the formation of highly reactive ultimate metabolites. As a consequence, genotoxic events occurred in BEAS-2B cells exposed to this OEM together with cell survival events, with possible harmful cell cycle deregulation. However, more studies are required to implement these observations and to contribute to better decipher the critical role of the organic fraction of air pollution-derived PM2.5Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Institut Pasteur de Lille
Université de Lille

Collections :

• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Submission date :

2022-02-02T10:23:51Z
2024-03-13T11:04:19Z