Title :

Immune monitoring in melanoma and urothelial cancer patients treated with anti-pd-1 immunotherapy and sbrt discloses tumor specific immune signatures

Author(s) :

Meireson, Annabel [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Tavernier, Simon J. [Auteur]
Vlaams Instituut voor Biotechnologie [Ghent, Belgique] [VIB]
Ghent University Hospital
Van Gassen, Sofie [Auteur]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Sundahl, Nora [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Demeyer, Annelies [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Spaas, Mathieu [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Kruse, Vibeke [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Ferdinande, Liesbeth [Auteur]
Ghent University Hospital
Van Dorpe, Jo [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Hennart, Benjamin [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Allorge, Delphine [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Haerynck, Filomeen [Auteur]
Ghent University Hospital
Decaestecker, Karel [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Rottey, Sylvie [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Saeys, Yvan [Auteur]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Ost, Piet [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Brochez, Lieve [Auteur]
Ghent University Hospital
Universiteit Gent = Ghent University = Université de Gand [UGENT]

Journal title :

Cancers

Abbreviated title :

Cancers (Basel)

Volume number :

13

Pages :

2630

Publication date :

2021-05-27

ISSN :

2072-6694

English keyword(s) :

immunotherapy
anti-PD-1
melanoma
urothelial cancer
immune monitoring
blood biomarkers

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

(1) Background: Blockade of the PD-1/PD-L1 pathway has revolutionized the oncology field in the last decade. However, the proportion of patients experiencing a durable response is still limited. In the current study, we ...
Show more >(1) Background: Blockade of the PD-1/PD-L1 pathway has revolutionized the oncology field in the last decade. However, the proportion of patients experiencing a durable response is still limited. In the current study, we performed an extensive immune monitoring in patients with stage III/IV melanoma and stage IV UC who received anti-PD-1 immunotherapy with SBRT. (2) Methods: In total 145 blood samples from 38 patients, collected at fixed time points before and during treatment, were phenotyped via high-parameter flow cytometry, luminex assay and UPLC-MS/MS. (3) Results: Baseline systemic immunity in melanoma and UC patients was different with a more prominent myeloid compartment and a higher neutrophil to lymphocyte ratio in UC. Proliferation (Ki67+) of CD8+ T-cells and of the PD-1+/PD-L1+ CD8+ subset at baseline correlated with progression free survival in melanoma. In contrast a higher frequency of PD-1/PD-L1 expressing non-proliferating (Ki67−) CD8+ and CD4+ T-cells before treatment was associated with worse outcome in melanoma. In UC, the expansion of Ki67+ CD8+ T-cells and of the PD-L1+ subset relative to tumor burden correlated with clinical outcome. (4) Conclusion: This study reveals a clearly different immune landscape in melanoma and UC at baseline, which may impact immunotherapy response. Signatures of proliferation in the CD8+ T-cell compartment prior to and early after anti-PD-1 initiation were positively correlated with clinical outcome in both cohorts. PD-1/PD-L1 expression on circulating immune cell subsets seems of clinical relevance in the melanoma cohort.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Institut Pasteur de Lille
Université de Lille

Collections :

• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Submission date :

2022-02-02T10:24:57Z
2022-05-24T12:34:04Z