Airway Administration of Flagellin Regulates ...
Document type :
Article dans une revue scientifique
DOI :
PMID :
Permalink :
Title :
Airway Administration of Flagellin Regulates the Inflammatory Response to Pseudomonas aeruginosa
Author(s) :
López-Gálvez, Raquel [Auteur]
Infectiologie et Santé Publique [ISP]
Fleurot, Isabelle [Auteur]
Infectiologie et Santé Publique [ISP]
Chamero, Pablo [Auteur]
Institut Français du Cheval et de l'Equitation [Saumur] [IFCE]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Trapp, Sascha [Auteur]
Infectiologie et Santé Publique [ISP]
Olivier, Michel [Auteur]
Infectiologie et Santé Publique [ISP]
Chevaleyre, Claire [Auteur]
Infectiologie et Santé Publique [ISP]
Barc, Céline [Auteur]
Plateforme d'Infectiologie Expérimentale [PFIE]
Riou, Mickael [Auteur]
Plateforme d'Infectiologie Expérimentale [PFIE]
Rossignol, Christelle [Auteur]
Infectiologie et Santé Publique [ISP]
Guillon, Antoine [Auteur]
Université de Tours [UT]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
Si-Tahar, Mustapha [Auteur]
Université de Tours [UT]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
May, Tobias [Auteur]
Barbry, Pascal [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Bähr, Andrea [Auteur]
Ludwig Maximilian University [Munich] = Ludwig Maximilians Universität München [LMU]
Klymiuk, Nikolai [Auteur]
Ludwig Maximilian University [Munich] = Ludwig Maximilians Universität München [LMU]
SIRARD, Jean-Claude [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Caballero, Ignacio [Auteur]
Infectiologie et Santé Publique [ISP]
Infectiologie et Santé Publique [ISP]
Fleurot, Isabelle [Auteur]
Infectiologie et Santé Publique [ISP]
Chamero, Pablo [Auteur]
Institut Français du Cheval et de l'Equitation [Saumur] [IFCE]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Trapp, Sascha [Auteur]
Infectiologie et Santé Publique [ISP]
Olivier, Michel [Auteur]
Infectiologie et Santé Publique [ISP]
Chevaleyre, Claire [Auteur]
Infectiologie et Santé Publique [ISP]
Barc, Céline [Auteur]
Plateforme d'Infectiologie Expérimentale [PFIE]
Riou, Mickael [Auteur]
Plateforme d'Infectiologie Expérimentale [PFIE]
Rossignol, Christelle [Auteur]
Infectiologie et Santé Publique [ISP]
Guillon, Antoine [Auteur]
Université de Tours [UT]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
Si-Tahar, Mustapha [Auteur]
Université de Tours [UT]
Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 [CEPR]
May, Tobias [Auteur]
Barbry, Pascal [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Bähr, Andrea [Auteur]
Ludwig Maximilian University [Munich] = Ludwig Maximilians Universität München [LMU]
Klymiuk, Nikolai [Auteur]
Ludwig Maximilian University [Munich] = Ludwig Maximilians Universität München [LMU]
SIRARD, Jean-Claude [Auteur]

Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Caballero, Ignacio [Auteur]
Infectiologie et Santé Publique [ISP]
Journal title :
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Pages :
378-389
Publisher :
American Thoracic Society
Publication date :
2021-10
ISSN :
1044-1549
English keyword(s) :
Pseudomonas aeruginosa
TLR5
cystic fibrosis
flagellin
inflammation
TLR5
cystic fibrosis
flagellin
inflammation
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Excessive lung inflammation and airway epithelial damage are hallmarks of human inflammatory lung diseases, such as cystic fibrosis (CF). Enhancement of innate immunity provides protection against pathogens while reducing ...
Show more >Excessive lung inflammation and airway epithelial damage are hallmarks of human inflammatory lung diseases, such as cystic fibrosis (CF). Enhancement of innate immunity provides protection against pathogens while reducing lung-damaging inflammation. However, the mechanisms underlying innate immunity-mediated protection in the lung remain mysterious, in part because of the lack of appropriate animal models for these human diseases. TLR5 (Toll-like receptor 5) stimulation by its specific ligand, the bacterial protein flagellin, has been proposed to enhance protection against several respiratory infectious diseases, although other cellular events, such as calcium signaling, may also control the intensity of the innate immune response. Here, we investigated the molecular events prompted by stimulation with flagellin and its role in regulating innate immunity in the lung of the pig, which is anatomically and genetically more similar to humans than rodent models. We found that flagellin treatment modulated NF-κB signaling and intracellular calcium homeostasis in airway epithelial cells. Flagellin pretreatment reduced the NF-κB nuclear translocation and the expression of proinflammatory cytokines to a second flagellin stimulus as well as to Pseudomonas aeruginosa infection. Moreover, in vivo administration of flagellin decreased the severity of P. aeruginosa-induced pneumonia. Then we confirmed these beneficial effects of flagellin in a pathological model of CF by using ex vivo precision-cut lung slices from a CF pigz model. These results provide evidence that flagellin treatment contributes to a better regulation of the inflammatory response in inflammatory lung diseases such as CF.Show less >
Show more >Excessive lung inflammation and airway epithelial damage are hallmarks of human inflammatory lung diseases, such as cystic fibrosis (CF). Enhancement of innate immunity provides protection against pathogens while reducing lung-damaging inflammation. However, the mechanisms underlying innate immunity-mediated protection in the lung remain mysterious, in part because of the lack of appropriate animal models for these human diseases. TLR5 (Toll-like receptor 5) stimulation by its specific ligand, the bacterial protein flagellin, has been proposed to enhance protection against several respiratory infectious diseases, although other cellular events, such as calcium signaling, may also control the intensity of the innate immune response. Here, we investigated the molecular events prompted by stimulation with flagellin and its role in regulating innate immunity in the lung of the pig, which is anatomically and genetically more similar to humans than rodent models. We found that flagellin treatment modulated NF-κB signaling and intracellular calcium homeostasis in airway epithelial cells. Flagellin pretreatment reduced the NF-κB nuclear translocation and the expression of proinflammatory cytokines to a second flagellin stimulus as well as to Pseudomonas aeruginosa infection. Moreover, in vivo administration of flagellin decreased the severity of P. aeruginosa-induced pneumonia. Then we confirmed these beneficial effects of flagellin in a pathological model of CF by using ex vivo precision-cut lung slices from a CF pigz model. These results provide evidence that flagellin treatment contributes to a better regulation of the inflammatory response in inflammatory lung diseases such as CF.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Source :
Submission date :
2022-02-09T02:31:08Z