Title :

Oxidation of 2,5-diformfylfuran to 2,5-furandicarboxylic acid catalyzed by candida antarctica lipase b immobilized in a cyclodextrin-templated mesoporous silica. The critical role of pore characteristics on the catalytic performance

Author(s) :

Decarpigny, Cedric [Auteur]
Bleta, Rudina [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181
Ponchel, Anne [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181
Monflier, Eric [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Journal title :

Colloids and surfaces. B, Biointerfaces

Abbreviated title :

Colloids Surf B Biointerfaces

Volume number :

200

Pages :

111606

Publication date :

2021-02-05

ISSN :

1873-4367

HAL domain(s) :

Chimie/Catalyse

English abstract : [en]

Hypothesis: Porous silica has been extensively used as suitable carrier for the immobilization of various enzymes. Randomly Methylated β-Cyclodextrin (RaMeβCD) has surface active properties and very high solubility in water ...
Show more >Hypothesis: Porous silica has been extensively used as suitable carrier for the immobilization of various enzymes. Randomly Methylated β-Cyclodextrin (RaMeβCD) has surface active properties and very high solubility in water and could therefore be used as template in the fabrication of silica particles with tunable pore size. Experiments: Silica particles were prepared by sol-gel process in alkaline medium with and without use of RaMeβCD. Lipase Bfrom Candida antarctica (CALB) was either incorporated within the pores of RaMeβCD-derived support or covalently attached on the surface of CD-free silica particles and its catalytic performance was assayed in the oxidation of 2,5-diformylfuran (DFF) to 2,5-furandicarboxylic acid (FDCA). Enzymatic reactors were characterized by N2-adsorption analysis, small angle XRD, TG/DSC experiments, ATR-FTIR spectroscopy, HR-TEM and LSCM, while reaction products were determined based on 1H NMR spectroscopy combined with HPLC. Findings: Results showed that the use of RaMeβCD as structure directing agent led to mesoporous silica composed of uniform 8 nm-sized particles with 11 nm-sized mesopores compatible with the dimensions of CALB (3.0 nm × 4.0 nm × 5.0 nm). Incorporation of CALB within the pores of RaMeβCD-derived silica caused almost a two-fold increase in specific activity after 7 h at 40 °C when compared to lipase immobilized on the surface of CD-free silica particles (33.2 μmol g-1 min-1vs. 14.4 μmol g-1 min-1). Moreover, the RaMeβCD-derived biocatalyst demonstrated enhanced operational stability during the recycling experiments, retaining more than 90% of its initial activity after five 24 h-reaction cycles. These findings open up new avenues for future research on the use of cyclodextrins in the development of enzyme-based nanoreactors.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
Centrale Lille
ENSCL
Univ. Artois
Université de Lille

Collections :

• Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Research team(s) :

Catalyse et chimie supramoléculaire (CASU)

Submission date :

2022-03-02T07:13:03Z
2023-12-15T19:50:58Z