Exposure to atmospheric Ag, TiO2, Ti and ...
Document type :
Article dans une revue scientifique: Article original
Title :
Exposure to atmospheric Ag, TiO2, Ti and SiO2 engineered nanoparticles modulates gut inflammatory response and microbiota in mice
Author(s) :
Guilloteau, Eva [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Djouina, Madjid [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Caboche, Ségolène [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Waxin, Christophe [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Deboudt, Karine [Auteur]
Laboratoire de Physico-Chimie de l'Atmosphère [LPCA]
Beury, Delphine [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Hot, David [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Pichavant, Muriel [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Dubuquoy, Laurent [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Launay, David [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Vignal, Cecile [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Choël, Marie [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Body-Malapel, Mathilde [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Djouina, Madjid [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Caboche, Ségolène [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Waxin, Christophe [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Deboudt, Karine [Auteur]
Laboratoire de Physico-Chimie de l'Atmosphère [LPCA]
Beury, Delphine [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Hot, David [Auteur]

Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Pichavant, Muriel [Auteur]

Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Dubuquoy, Laurent [Auteur]

Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Launay, David [Auteur]

Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Vignal, Cecile [Auteur]

Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Choël, Marie [Auteur]

Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Body-Malapel, Mathilde [Auteur]

Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Journal title :
Ecotoxicology and Environmental Safety
Pages :
113442
Publisher :
Elsevier
Publication date :
2022-05
ISSN :
0147-6513
English keyword(s) :
Engineered nanoparticles
Inhalation
Inflammation
Colitis
Organoid
Mixture
Microbiome
Digestive tract
Intestin
Inhalation
Inflammation
Colitis
Organoid
Mixture
Microbiome
Digestive tract
Intestin
HAL domain(s) :
Sciences du Vivant [q-bio]/Biologie animale
Sciences du Vivant [q-bio]
Chimie/Chimie analytique
Sciences du Vivant [q-bio]
Chimie/Chimie analytique
English abstract : [en]
The development of nanotechnologies is leading to greater abundance of engineered nanoparticles (EN) in the environment, including in the atmospheric air. To date, it has been shown that the most prevalent EN found in the ...
Show more >The development of nanotechnologies is leading to greater abundance of engineered nanoparticles (EN) in the environment, including in the atmospheric air. To date, it has been shown that the most prevalent EN found in the air are silver (Ag), titanium dioxide (TiO2), titanium (Ti), and silicon dioxide (SiO2). As the intestinal tract is increasingly recognized as a target for adverse effects induced by inhalation of air particles, the aim of this studywas to assess the impact of these 4 atmospheric EN on intestinal inflammation and microbiota. We assessed the combined toxicity effects of Ag, Ti, TiO2, and SiO2 following a 28-day inhalation protocol in male and female mice. In distal and proximal colon, and in jejunum, EN mixture inhalation did not induce overt histological damage, but led to a significant modulation of inflammatory cytokine transcript abundance, including downregulation of Tnfα, Ifnγ, Il1β, Il17a, Il22, IL10, and Cxcl1 mRNA levels in male jejunum. A dysbiosis was observed in cecal microbiota of male and female mice exposed to the EN mixture, characterized by sex-dependent modulations of specific bacterial taxa, as well as sex-independent decreased abundance of the Eggerthellaceae family.Under dextran sodium sulfate-induced inflammatory conditions, exposure to the EN mixture increased the development of colitis in both male and female mice. Moreover, the direct dose-response effects of individual and mixed EN on gut organoids was studied and Ag, TiO2, Ti, SiO2, and EN mixture were found to generate specific inflammatory responses in the intestinal epithelium. These results indicate that the 4 most prevalent atmospheric EN could have the ability to disturb intestinal homeostasis through direct modulation of cytokine expression in gut epithelium, and by altering the inflammatory response and microbiota composition following inhalationShow less >
Show more >The development of nanotechnologies is leading to greater abundance of engineered nanoparticles (EN) in the environment, including in the atmospheric air. To date, it has been shown that the most prevalent EN found in the air are silver (Ag), titanium dioxide (TiO2), titanium (Ti), and silicon dioxide (SiO2). As the intestinal tract is increasingly recognized as a target for adverse effects induced by inhalation of air particles, the aim of this studywas to assess the impact of these 4 atmospheric EN on intestinal inflammation and microbiota. We assessed the combined toxicity effects of Ag, Ti, TiO2, and SiO2 following a 28-day inhalation protocol in male and female mice. In distal and proximal colon, and in jejunum, EN mixture inhalation did not induce overt histological damage, but led to a significant modulation of inflammatory cytokine transcript abundance, including downregulation of Tnfα, Ifnγ, Il1β, Il17a, Il22, IL10, and Cxcl1 mRNA levels in male jejunum. A dysbiosis was observed in cecal microbiota of male and female mice exposed to the EN mixture, characterized by sex-dependent modulations of specific bacterial taxa, as well as sex-independent decreased abundance of the Eggerthellaceae family.Under dextran sodium sulfate-induced inflammatory conditions, exposure to the EN mixture increased the development of colitis in both male and female mice. Moreover, the direct dose-response effects of individual and mixed EN on gut organoids was studied and Ag, TiO2, Ti, SiO2, and EN mixture were found to generate specific inflammatory responses in the intestinal epithelium. These results indicate that the 4 most prevalent atmospheric EN could have the ability to disturb intestinal homeostasis through direct modulation of cytokine expression in gut epithelium, and by altering the inflammatory response and microbiota composition following inhalationShow less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Source :
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