How agarose gels surrounding plga implants limit swelling and slow down drug release

Bassand, Celine; Verin, Jérémy; Lamatsch, M.; Siepmann, Florence; Siepmann, Juergen

Document type :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.jconrel.2022.01.028

PMID :

35085697

Permalink :

http://hdl.handle.net/20.500.12210/73605

Title :

How agarose gels surrounding plga implants limit swelling and slow down drug release

Author(s) :

Bassand, Celine [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008
Verin, Jérémy [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008
Lamatsch, M. [Auteur]
Siepmann, Florence [Auteur]

Advanced Drug Delivery Systems (ADDS) - U1008
Siepmann, Juergen [Auteur]

Advanced Drug Delivery Systems (ADDS) - U1008

Journal title :

Journal of Controlled Release

Abbreviated title :

J Control Release

Volume number :

343

Pages :

255-266

Publication date :

2022-01-24

ISSN :

1873-4995

English keyword(s) :

PLGA implant
release mechanism
swelling
ibuprofen
hydrogel

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The aim of this study was to better understand to which extent and in which way the presence of an agarose gel (mimicking living tissue) around a PLGA [poly(lactic-co-glycolic acid)] implant affects the resulting drug ...
Show more >The aim of this study was to better understand to which extent and in which way the presence of an agarose gel (mimicking living tissue) around a PLGA [poly(lactic-co-glycolic acid)] implant affects the resulting drug release kinetics. Ibuprofen-loaded implants were prepared by hot melt extrusion. Drug release was measured upon exposure to phosphate buffer pH 7.4 in Eppendorf tubes, as well as upon inclusion into an agarose gel which was exposed to phosphate buffer pH 7.4 in an Eppendorf tube or in a transwell plate. Dynamic changes in the implants' dry & wet mass and dimensions were monitored gravimetrically and by optical macroscopy. Implant erosion and polymer degradation were observed by SEM and GPC. Different pH indicators were used to measure pH changes in the bulk fluids, gels and within the implants during drug release. Ibuprofen release was bi-phasic in all cases: A zero order release phase (~20% of the dose) was followed by a more rapid, final drug release phase. Interestingly, the presence of the hydrogel delayed the onset of the 2nd release phase. This could be attributed to the sterical hindrance of implant swelling: After a certain lag time, the degrading PLGA matrix becomes sufficiently hydrophilic and mechanically instable to allow for the penetration of substantial amounts of water into the system. This fundamentally changes the conditions for drug release: The latter becomes much more mobile and is more rapidly released. A gel surrounding the implant mechanically hinders system swelling and, thus, slows down drug release. These observations also strengthen the hypothesis of the "orchestrating" role of PLGA swelling for the control of drug release and can help developing more realistic in vitro release set-ups.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

Advanced Drug Delivery Systems (ADDS) - U1008

Submission date :

2022-04-13T07:12:02Z
2024-02-16T10:34:23Z
2024-04-02T13:23:13Z
2024-04-29T07:49:37Z

Files

Bassand_JCR_343_255-266.pdf
Version finale acceptée pour publication (postprint)
Open access
Access the document

Version history

Version	Link	Modification date
5	20.500.12210/73605*	2024-04-29T07:49:07Z
4	20.500.12210/73605.4	2024-04-29T07:45:41Z
3	20.500.12210/73605.3	2024-04-02T13:21:45Z
2	20.500.12210/73605.2	2024-02-16T10:27:40Z
1	20.500.12210/73605.1	2022-04-13T07:12:02Z

*Selected version

How agarose gels surrounding plga implants ... BibTeX CSV Excel RIS

Files

Version history

How agarose gels surrounding plga implants ...

BibTeX

CSV

Excel

RIS