Title :

Determination of Glucan Chain Length Distribution of Glycogen using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method.

Author(s) :

Fermont, Lea [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Szydlowski, Nicolas [Auteur]
Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290
Colleoni, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Journal title :

Journal of visualized experiments : JoVE

Abbreviated title :

J Vis Exp

Publication date :

2022-03-31

ISSN :

1940-087X

English keyword(s) :

Electrophoresis
Glucans
Glucosides
Glycogen
Polysaccharides

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Glycogen particles are branched polysaccharides composed of linear chains of glucosyl units linked by α-1,4 glucoside bonds. The latter are attached to each other by α-1,6 glucoside linkages, referred to as branch points. ...
Show more >Glycogen particles are branched polysaccharides composed of linear chains of glucosyl units linked by α-1,4 glucoside bonds. The latter are attached to each other by α-1,6 glucoside linkages, referred to as branch points. Among the different forms of carbon storage (i.e., starch, β-glucan), glycogen is probably one of the oldest and most successful storage polysaccharides found across the living world. Glucan chains are organized so that a large amount of glucose can quickly be stored or fueled in a cell when needed. Numerous complementary techniques have been developed over the last decades to solve the fine structure of glycogen particles. This article describes Fluorophore-Assisted Carbohydrate Electrophoresis (FACE). This method quantifies the population of glucan chains that compose a glycogen particle. Also known as chain length distribution (CLD), this parameter mirrors the particle size and the percentage of branching. It is also an essential requirement for the mathematical modeling of glycogen biosynthesis.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

ANR Project :

Tester l'hypothèse du ménage à trois

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Génétique microbienne
Plant Storage Polysaccharides

Submission date :

2022-05-12T10:11:33Z
2022-05-12T14:02:39Z
2022-05-25T08:28:58Z