Title :

One-Step Next-Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

Author(s) :

Villarese, Patrick [Auteur]
CHU Necker - Enfants Malades [AP-HP]
Abdo, Chrystelle [Auteur]
CHU Necker - Enfants Malades [AP-HP]
Bertrand, Matthieu [Auteur]
CHU Necker - Enfants Malades [AP-HP]
Thonier, Florian [Auteur]
Institut National de Recherche en Informatique et en Automatique [Inria]
Giraud, Mathieu [Auteur]
Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 [CRIStAL]
Salson, Mikael [Auteur]
Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 [CRIStAL]
Macintyre, Elizabeth [Auteur]
CHU Necker - Enfants Malades [AP-HP]

Scientific editor(s) :

Anton W. Langerak

Book title :

Immunogenetics. Methods and Protocols

Publisher :

Springer

Publication date :

2022

ISBN :

978-1-0716-2115-8

English keyword(s) :

Next-generation sequencing
One step
T cell receptor
B cell receptor

HAL domain(s) :

Sciences du Vivant [q-bio]/Cancer
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Hématologie
Sciences du Vivant [q-bio]/Immunologie/Immunité adaptative
Informatique [cs]/Bio-informatique [q-bio.QM]

English abstract : [en]

Within the EuroClonality-NGS group, immune repertoire analysis for target identification in lymphoid malignancies was initially developed using two-stage amplicon approaches, essentially as a progressive modification of ...
Show more >Within the EuroClonality-NGS group, immune repertoire analysis for target identification in lymphoid malignancies was initially developed using two-stage amplicon approaches, essentially as a progressive modification of preceding methods developed for Sanger sequencing. This approach has, however, limitations with respect to sample handling, adaptation to automation, and risk of contamination by amplicon products. We therefore developed one-step PCR amplicon methods with individual barcoding for batched analysis for IGH, IGK, TRD, TRG, and TRB rearrangements, followed by Vidjil-based data analysis.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL