Title :

Universal sample preparation unlocking multimodal molecular tissue imaging

Author(s) :

Dannhorn, Andreas [Auteur]
Kazanc, Emine [Auteur]
Ling, Stephanie [Auteur]
Nikula, Chelsea J. [Auteur]
Karali, Evdoxia [Auteur]
Serra, Maria Paola [Auteur]
Vorng, Jean-Luc [Auteur]
Inglese, Paolo [Auteur]
Maglennon, Gareth [Auteur]
Hamm, Gregory [Auteur]
Swales, John [Auteur]
Strittmatter, Nicole [Auteur]
Barry, Simon T. [Auteur]
Sansom, Owen J. [Auteur]
Poulogiannis, George [Auteur]
Bunch, Josephine [Auteur]
Goodwin, Richard J. A. [Auteur]
Takats, Zoltan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Journal title :

Analytical Chemistry

Abbreviated title :

Anal. Chem.

Volume number :

92

Pages :

-

Publication date :

2020-08-18

ISSN :

0003-2700

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the ...
Show more >A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the low temperature embedding of fresh frozen specimens into a hydrogel matrix composed of hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidone (PVP) and sectioning using a cryomicrotome. The hydrogel was expected not to interfere with standard tissue characterization methods, histologically or analytically. We assessed the compatibility of this protocol with various mass spectrometric imaging methods including matrix-assisted laser desorption ionization (MALDI), desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS). We also demonstrated the suitability of the universal protocol for extraction based molecular biology techniques such as rt-PCR. The integration of multiple analytical modalities through this universal sample preparation protocol offers the ability to study tissues at a systems biology level and directly linking results to tissue morphology and cellular phenotype.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:57:54Z