Title :

Arid1a associates with lymphoid-restricted transcription factors and has an essential role in t cell development

Author(s) :

Astori, Audrey [Auteur]
Princess Margaret Hospital
Tingvall-Gustafsson, Johanna [Auteur]

Kuruvilla, Jacob [Auteur]
Linköping University [LIU]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Sunnerhagen, Maria [Auteur]
Linköping University [LIU]
Ahsberg, Josefine [Auteur]
Linköping University [LIU]
Ungerback, Jonas [Auteur]

Strid, Tobias [Auteur]
Linköping University [LIU]
Sigvardsson, Mikael [Auteur]

Raught, Brian [Auteur]
Princess Margaret Hospital
Somasundaram, Rajesh [Auteur]
Linköping University [LIU]

Journal title :

Journal of immunology (Baltimore, Md. . 1950)

Abbreviated title :

J. Immunol.

Volume number :

205

Pages :

1419–1432

Publication date :

2020-09-01

ISSN :

0022-1767

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Maturation of lymphoid cells is controlled by the action of stage and lineage-restricted transcription factors working in concert with the general transcription and chromatin remodeling machinery to regulate gene expression. ...
Show more >Maturation of lymphoid cells is controlled by the action of stage and lineage-restricted transcription factors working in concert with the general transcription and chromatin remodeling machinery to regulate gene expression. To better understand this functional interplay, we used Biotin Identification in human embryonic kidney cells to identify proximity interaction partners for GATA3, TCF7 (TCF1), SPI1, HLF, IKZF1, PAX5, ID1, and ID2. The proximity interaction partners shared among the lineage-restricted transcription factors included ARID1a, a BRG1-associated factor complex component. CUT&RUN analysis revealed that ARID1a shared binding with TCF7 and GATA3 at a substantial number of putative regulatory elements in mouse T cell progenitors. In support of an important function for ARID1a in lymphocyte development, deletion of Arid1a in early lymphoid progenitors in mice resulted in a pronounced developmental arrest in early T cell development with a reduction of CD4(+)CD8(+) cells and a 20-fold reduction in thymic cellularity. Exploring gene expression patterns in DN3 cells from Wt and Arid1a-deficient mice suggested that the developmental block resided in the DN3a to DN3b transition, indicating a deficiency in beta-selection. Our work highlights the critical importance of functional interactions between stage and lineage-restricted factors and the basic transcription machinery during lymphocyte differentiation.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:57:56Z
2023-04-05T07:52:58Z