Title :

A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program

Author(s) :

Giudetti, Anna Maria [Auteur]
De Domenico, Stefania [Auteur]
Ragusa, Andrea [Auteur]
Lunetti, Paola [Auteur]
Gaballo, Antonio [Auteur]
Franck, Julien [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Simeone, Pasquale [Auteur]
Nicolardi, Giuseppe [Auteur]
De Nuccio, Francesco [Auteur]
Santino, Angelo [Auteur]
Capobianco, Loredana [Auteur]
Lanuti, Paola [Auteur]
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Maffia, Michele [Auteur]
Vergara, Daniele [Auteur]

Journal title :

Biochimica et biophysica acta. Molecular and cell biology of lipids

Abbreviated title :

Biochim Biophys Acta Mol Cell Biol Lipids

Publication date :

2018-12-19

ISSN :

1879-2618

Keyword(s) :

Metabolism
Epithelial mesenchymal transition
beta-Catenin
Lipids
Mass spectrometry

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Several studies have identified a specific metabolic program that is associated with the process of epithelial-mesenchymal transition (EMT). Whereas much is known about the association between glucose metabolism and EMT, ...
Show more >Several studies have identified a specific metabolic program that is associated with the process of epithelial-mesenchymal transition (EMT). Whereas much is known about the association between glucose metabolism and EMT, the contribution of lipid metabolism is not still completely understood. Here, we studied epithelial and mesenchymal breast cancer cells by proteomic and lipidomic approaches and identified significant differences that characterised these models concerning specific metabolic enzymes and metabolites including fatty acids and phospholipids. Higher levels of monounsaturated fatty acids together with increased expression of enzymes of de novo fatty acid synthesis is the distinct signature of epithelial with respect to mesenchymal cells that, on the contrary, show reduced lipogenesis, higher polyunsaturated fatty acids level and increased expression of genes involved in the triacylglycerol (TAG) synthesis and lipid droplets formation. In the mesenchymal model, the diacylglycerol acyltransferase (DGAT)-1 appears to be the major enzyme involved in TAG synthesis and inhibition of DGAT1, but not DGAT2, drastically reduces the incorporation of labeled palmitate into TAG. Moreover, knockdown of beta-catenin demonstrated that this metabolic phenotype in under the control of a network of transcriptional factors and that beta-catenin has a specific role in the regulation of lipid metabolism in mesenchymal cells.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:58:58Z