Title :

Distinct protein expression networks are activated in microglia cells after stimulation with ifn-? and il-4

Author(s) :

Vergara, Daniele [Auteur]
Università del Salento = University of Salento [Lecce]
Nigro, Annamaria [Auteur]
IRCCS San Raffaele Scientific Institute [Milan, Italie]
Romano, Alessandro [Auteur]
IRCCS San Raffaele Scientific Institute [Milan, Italie]
De Domenico, Stefania [Auteur]
Institute of Sciences of Food Production [ISPA]
Damato, Marina [Auteur]
Università del Salento = University of Salento [Lecce]
Franck, Julien [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Coricciati, Chiara [Auteur]
Università del Salento = University of Salento [Lecce]
Wisztorski, Maxence [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Cardon, Tristan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Quattrini, Angelo [Auteur]
IRCCS Ospedale San Raffaele [Milan, Italy]
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Furlan, Roberto [Auteur]
IRCCS San Raffaele Scientific Institute [Milan, Italie]
Maffia, Michele [Auteur]
Università del Salento = University of Salento [Lecce]

Journal title :

Cells

Abbreviated title :

Cells

Volume number :

8

Pages :

580

Publication date :

2019-06-12

ISSN :

2073-4409

Keyword(s) :

microglia
microglia plasticity
IL-4
mass spectrometry
proteomics
IFN-gamma

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Microglia cells are the primary immune population of the central nervous system with a role in the regulation of several physiological and pathological conditions. Upon appropriate stimulation, microglia cells can be ...
Show more >Microglia cells are the primary immune population of the central nervous system with a role in the regulation of several physiological and pathological conditions. Upon appropriate stimulation, microglia cells can be polarized in a pro-inflammatory M1-like or anti-inflammatory M2-like status. Biological processes and pathways engaged in microglia polarization are starting to be elucidated. To help clarify this, we used a liquid chromatography-mass spectrometry (LC-MS/MS) label free approach to characterize the proteomic profile of human microglia cell line (CHME-5) stimulated with gamma-interferon (IFN-gamma) and interleukin-4 (IL-4) to induce a M1 or M2 phenotype, respectively. Outside the classical M1/M2 polarization markers, the M1 status appears to center around the activation of a classical inflammatory response and through the activation of multiple signaling pathways. M2 polarization resulted in a different pattern of protein modulation related to RNA and cellular metabolic processes. Together, our findings provide information regarding the protein changes specific to M1 and M2 activation states, and potentially link the polarization of microglia cells to the acquisition of a specific proteomic profile.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:59:03Z
2023-01-18T10:11:30Z