Mechanisms of innate events during skin ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Mechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine
Author(s) :
Gonnet, Jessica [Auteur]
Poncelet, Lauranne [Auteur]
Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS]
Meriaux, Celine [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Goncalves, Elena [Auteur]
Weiss, Lina [Auteur]
Tchitchek, Nicolas [Auteur]
Pedruzzi, Eric [Auteur]
Soria, Angele [Auteur]
Boccara, David [Auteur]
Vogt, Annika [Auteur]
Bonduelle, Olivia [Auteur]
Hamm, Gregory [Auteur]
Ait-Belkacem, Rima [Auteur]
Stauber, Jonathan [Auteur]
Fournier, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Wisztorski (Admin), Maxence [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Combadiere, Behazine [Auteur]
Poncelet, Lauranne [Auteur]
Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS]
Meriaux, Celine [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Goncalves, Elena [Auteur]
Weiss, Lina [Auteur]
Tchitchek, Nicolas [Auteur]
Pedruzzi, Eric [Auteur]
Soria, Angele [Auteur]
Boccara, David [Auteur]
Vogt, Annika [Auteur]
Bonduelle, Olivia [Auteur]
Hamm, Gregory [Auteur]
Ait-Belkacem, Rima [Auteur]
Stauber, Jonathan [Auteur]
Fournier, Isabelle [Auteur]

Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Wisztorski (Admin), Maxence [Auteur]

Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Combadiere, Behazine [Auteur]
Journal title :
Journal of Proteomics
Abbreviated title :
J Proteomics
Volume number :
216
Pages :
103670
Publisher :
Elsevier
Publication date :
2020-01-25
ISSN :
1874-3919
English keyword(s) :
Innate immunity
Biomarkers
Skin
Intradermal vaccination
Mass spectrometry
Biomarkers
Skin
Intradermal vaccination
Mass spectrometry
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Immunologie
Sciences du Vivant [q-bio]/Immunologie
English abstract : [en]
The skin plays a crucial role in host defences against microbial attack and the innate cells must provide the immune system with sufficient information to organize these defences. This unique feature makes the skin a ...
Show more >The skin plays a crucial role in host defences against microbial attack and the innate cells must provide the immune system with sufficient information to organize these defences. This unique feature makes the skin a promising site for vaccine administration. Although cellular innate immune events during vaccination have been widely studied, initial events remain poorly understood. Our aim is to determine molecular biomarkers of skin innate reaction after intradermal (i.d.) immunization. Using an ex vivo human explant model from healthy donors, we investigated by NanoLC-MS/MS analysis and MALDI-MSI imaging, to detect innate molecular events (lipids, metabolites, proteins) few hours after i.d. administration of seasonal trivalent influenza vaccine (TIV). This multimodel approach allowed to identify early molecules differentially expressed in dermal and epidermal layers at 4 and 18h after TIV immunization compared with control PBS. In the dermis, the most relevant network of proteins upregulated were related to cell-to-cell signalling and cell trafficking. The molecular signatures detected were associated with chemokines such as CXCL8, a chemoattractant of neutrophils. In the epidermis, the most relevant networks were associated with activation of antigen-presenting cells and related to CXCL10. Our study proposes a novel step-forward approach to identify biomarkers of skin innate reaction. SIGNIFICANCE: To our knowledge, there is no study analyzing innate molecular reaction to vaccines at the site of skin immunization. What is known on skin reaction is based on macroscopic (erythema, redness...), microscopic (epidermal and dermal tissues) and cellular events (inflammatory cell infiltrate). Therefore, we propose a multimodal approach to analyze molecular events at the site of vaccine injection on skin tissue. We identified early molecular networks involved biological functions such cell migration, cell-to-cell interaction and antigen presentation, validated by chemokine expression, in the epidermis and dermis, then could be used as early indicator of success in immunization.Show less >
Show more >The skin plays a crucial role in host defences against microbial attack and the innate cells must provide the immune system with sufficient information to organize these defences. This unique feature makes the skin a promising site for vaccine administration. Although cellular innate immune events during vaccination have been widely studied, initial events remain poorly understood. Our aim is to determine molecular biomarkers of skin innate reaction after intradermal (i.d.) immunization. Using an ex vivo human explant model from healthy donors, we investigated by NanoLC-MS/MS analysis and MALDI-MSI imaging, to detect innate molecular events (lipids, metabolites, proteins) few hours after i.d. administration of seasonal trivalent influenza vaccine (TIV). This multimodel approach allowed to identify early molecules differentially expressed in dermal and epidermal layers at 4 and 18h after TIV immunization compared with control PBS. In the dermis, the most relevant network of proteins upregulated were related to cell-to-cell signalling and cell trafficking. The molecular signatures detected were associated with chemokines such as CXCL8, a chemoattractant of neutrophils. In the epidermis, the most relevant networks were associated with activation of antigen-presenting cells and related to CXCL10. Our study proposes a novel step-forward approach to identify biomarkers of skin innate reaction. SIGNIFICANCE: To our knowledge, there is no study analyzing innate molecular reaction to vaccines at the site of skin immunization. What is known on skin reaction is based on macroscopic (erythema, redness...), microscopic (epidermal and dermal tissues) and cellular events (inflammatory cell infiltrate). Therefore, we propose a multimodal approach to analyze molecular events at the site of vaccine injection on skin tissue. We identified early molecular networks involved biological functions such cell migration, cell-to-cell interaction and antigen presentation, validated by chemokine expression, in the epidermis and dermis, then could be used as early indicator of success in immunization.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
INSERM
Université de Lille
Université de Lille
Collections :
Submission date :
2022-06-15T13:59:24Z
2023-03-10T10:56:45Z
2023-03-10T10:56:45Z
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