Titre :

Persistence of coxsackievirus b4 in pancreatic ? cells disturbs insulin maturation, pattern of cellular proteins, and dna methylation

Auteur(s) :

Nekoua, Magloire Pandoua [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Bertin, Antoine [Auteur]
Pathogenèse virale du diabète de type 1 - ULR 3610
Sane, Famara [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pathogenèse virale du diabète de type 1 - ULR 3610
Gimeno, jean-pascal [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Engelmann, Ilka [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pathogenèse virale du diabète de type 1 - ULR 3610
Alidjinou, Enagnon Kazali [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Hober, Didier [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pathogenèse virale du diabète de type 1 - ULR 3610

Titre de la revue :

Microorganisms

Nom court de la revue :

Microorganisms

Numéro :

9

Pagination :

1125

Éditeur :

MDPI

Date de publication :

2021-05-22

ISSN :

2076-2607

Mot(s)-clé(s) :

type 1 diabetes
coxsackievirus B4
persistence
insulin
pro-hormone convertase 2
DNA methylation
pancreatic beta cell
in vitro
INS-1 cell line

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Coxsackievirus-B4 (CV-B4) can persist in pancreatic cell lines and impair the phenoytpe and/or gene expressions in these cells; however, the models used to study this phenomenon did not produce insulin. Therefore, we ...
Lire la suite >Coxsackievirus-B4 (CV-B4) can persist in pancreatic cell lines and impair the phenoytpe and/or gene expressions in these cells; however, the models used to study this phenomenon did not produce insulin. Therefore, we investigated CV-B4 persistence and its consequences in insulin-producing pancreatic beta cells. The insulin-secreting rat beta cell line, INS-1, was infected with CV-B4. After lysis of a large part of the cell layer, the culture was still maintained and no additional cytopathic effect was observed. The amount of insulin in supernatants of cell cultures persistently infected with CV-B4 was not affected by the infection; in fact, a larger quantity of proinsulin was found. The mRNA expression of pro-hormone convertase 2, an enzyme involved in the maturation of proinsulin into insulin and studied using real-time reverse transcription-polymerase chain reaction, was inhibited in infected cultures. Further, the pattern of 47 cell proteins analyzed using Shotgun mass spectrometry was significantly modified. The DNA of persistently infected cell cultures was hypermethylated unlike that of controls. The persistent infection of INS-1 cells with CV-B4 had a deep impact on these cells, especially on insulin metabolism. Cellular changes caused by persistent CV-B4 infection of beta cells can play a role in type 1 diabetes pathogenesis.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

CHU Lille
INSERM
Université de Lille

Collections :

• Laboratoire de virologie - ULR 3610
• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Date de dépôt :

2022-06-15T14:00:18Z
2023-01-25T09:38:30Z