Title :

Glycopeptidolipid glycosylation controls surface properties and pathogenicity in Mycobacterium abscessus

Author(s) :

Daher, Wassim [Auteur]
Institut de Recherche en Infectiologie de Montpellier [IRIM]
Leclercq, Louis-David [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Johansen, Matt D. [Auteur]
Institut de Recherche en Infectiologie de Montpellier [IRIM]
Hamela, Claire [Auteur]
Institut de Recherche en Infectiologie de Montpellier [IRIM]
Karam, Jona [Auteur]
Institut de Recherche en Infectiologie de Montpellier [IRIM]
Trivelli, Xavier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Nigou, Jérôme [Auteur]
Institut de pharmacologie et de biologie structurale [IPBS]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Kremer, Laurent [Auteur]
Institut de Recherche en Infectiologie de Montpellier [IRIM]

Journal title :

Cell Chemical Biology

Volume number :

29

Pages :

910-924.e7

Publisher :

Elsevier BV

Publication date :

2022-05

ISSN :

2451-9456

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Mycobacterium abscessus is an emerging and difficult-to-manage mycobacterial species that exhibits smooth (S) or rough (R) morphotypes. Disruption of glycopeptidolipid (GPL) production results in transition from S to R and ...
Show more >Mycobacterium abscessus is an emerging and difficult-to-manage mycobacterial species that exhibits smooth (S) or rough (R) morphotypes. Disruption of glycopeptidolipid (GPL) production results in transition from S to R and severe lung disease. A structure-activity relationship study was undertaken to decipher the role of GPL glycosylation in morphotype transition and pathogenesis. Deletion of gtf3 uncovered the prominent role of the extra rhamnose in enhancing mannose receptor-mediated internalization of M. abscessus by macrophages. In contrast, the absence of the 6-deoxy-talose and the first rhamnose in mutants lacking gtf1 and gtf2, respectively, affected M abscessus phagocytosis but also resulted in the S-to-R transition. Strikingly, gtf1 and gtf2 mutants displayed a strong propensity to form cords and abscesses in zebrafish, leading to robust and lethal infection. Together, these results underscore the importance and differential contribution of GPL monosaccharides in promoting virulence and infection outcomes.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Chemical Glycobiology

Submission date :

2022-07-28T14:04:19Z
2022-08-24T12:14:04Z
2022-10-03T10:13:22Z
2022-11-07T15:34:59Z