Title :

DHA-containing phospholipids control membrane fusion and transcellular tunnel dynamics

Author(s) :

Tsai, Meng-Chen [Auteur]
Toxines bactériennes - Bacterial Toxins
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Fleuriot, Lucile [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Janel, Sébastien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Gonzalez-Rodriguez, David [Auteur]
Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes [LCP-A2MC]
Morel, Camille [Auteur]
Toxines bactériennes - Bacterial Toxins
Mettouchi, Amel [Auteur]
Toxines bactériennes - Bacterial Toxins
Debayle, Delphine [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Dallongeville, Stéphane [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Olivo-Marin, Jean-Christophe [Auteur]
Analyse d'images biologiques - Biological Image Analysis [BIA]
Antonny, Bruno [Auteur]
Institut de pharmacologie moléculaire et cellulaire [IPMC]
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lemichez, Emmanuel [Auteur correspondant]
Toxines bactériennes - Bacterial Toxins
Barelli, Hélène [Auteur correspondant]
Institut de pharmacologie moléculaire et cellulaire [IPMC]

Journal title :

Journal of Cell Science

Pages :

jcs259119

Publisher :

Company of Biologists

Publication date :

2022-03-01

ISSN :

0021-9533

English keyword(s) :

Polyunsaturated phospholipids
Docosahexaenoic acid
DHA
Membrane fusion
Large-scale membrane dynamics
Endothelium
Transendothelial cell macroaperture
Bacterial toxin
Actin cytoskeleton

HAL domain(s) :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC]
Sciences du Vivant [q-bio]/Biologie cellulaire/Interactions cellulaires [q-bio.CB]

English abstract : [en]

Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (DHA-PLs) in physiology. Here, we investigated the impact of DHA-PLs on ...
Show more >Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (DHA-PLs) in physiology. Here, we investigated the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) triggered by RhoA inhibition-associated cell spreading. Lipidomic analyses showed that human umbilical vein endothelial cells (HUVECs) subjected to a DHA diet undergo a 6-fold enrichment in DHA-PLs at the plasma membrane (PM) at the expense of monounsaturated oleic acid-containing PLs (OA-PLs). Consequently, DHA-PL enrichment at the PM induces a reduction in cell thickness and shifts cellular membranes towards a permissive mode of membrane fusion for transcellular tunnel initiation. We provide evidence that a global homeostatic control of membrane tension and cell cortex rigidity minimizes overall changes of TEM area through a decrease of TEM size and lifetime. Conversely, low DHA-PL levels at the PM lead to the opening of unstable and wider TEMs. Together, this provides evidence that variations of DHA-PL levels in membranes affect cell biomechanical properties.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie
Vers la prévention de dysfonctions vasculaires et la délivrance de médicaments au travers des endothelia
Integrative Biology of Emerging Infectious Diseases
Développment d'une infrastructure française distribuée coordonnée

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL