Titre :

Ultrastructural modifications induced by SARS-CoV-2 in Vero cells : a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release

Auteur(s) :

Eymieux, Sébastien [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]
Rouillé, Yves [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Terrier, Olivier [Auteur]
Virology and human respiratory Pathologies - Virology and human respiratory Pathologies [CIRI] [CIRI-VirPath]
Seron, Karin [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Blanchard, Emmanuelle [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]
Rosa-Calatrava, Manuel [Auteur]
Virology and human respiratory Pathologies - Virology and human respiratory Pathologies [CIRI] [CIRI-VirPath]
Dubuisson, Jean [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Belouzard, Sandrine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Roingeard, Philippe [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]

Titre de la revue :

Cellular and Molecular Life Sciences

Éditeur :

Springer Verlag

Date de publication :

2021-01-15

ISSN :

1420-682X

Mot(s)-clé(s) en anglais :

Covid-19
SARS-CoV-2
virus/cell interactions
electron microscopy

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Biologie cellulaire/Interactions cellulaires [q-bio.CB]

Résumé en anglais : [en]

Many studies on SARS-CoV-2 have been performed over short time scale, but few have focused on the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis of the ultrastructure of ...
Lire la suite >Many studies on SARS-CoV-2 have been performed over short time scale, but few have focused on the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis of the ultrastructure of SARS-CoV-2-infected cells. Early infection events were characterized by the presence of clusters of single-membrane vesicles and stacks of membranecontaining nuclear pores called annulate lamellae (AL). A large network of host cell-derived organelles transformed into virus factories was subsequently observed in the cells. As previously described for other RNA viruses, these replication factories consisted of doublemembrane vesicles (DMVs) located close to the nucleus. Viruses released at the cell surface by exocytosis harbored the typical crown of spike proteins, but viral particles without spikes were also observed in intracellular compartments, possibly reflecting incorrect assembly or a cell degradation process.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

Valeur pronostique des auto-anticorps ANTi-Interféron de type I chez les patients pris en charge pour un syndrome de détresse respiratoire aiguë associé au COVID-19 : une étude de cohorte observationnelle multicentriq

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL