Titre :

One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae

Auteur(s) :

Maeno, Toshiki [Auteur]
Tokyo University of Pharmacy and Life Sciences [TUPLS]
Yamakawa, Yuki [Auteur]
Tokyo University of Pharmacy and Life Sciences [TUPLS]
Takiyasu, Yohei [Auteur]
Tokyo University of Pharmacy and Life Sciences [TUPLS]
Miyauchi, Hiroki [Auteur]
Tokyo University of Pharmacy and Life Sciences [TUPLS]
Nakamura, Yasunori [Auteur]
Akita University
Ono, Masami [Auteur]
Akita University
Ozaki, Noriaki [Auteur]
Akita University
Utsumi, Yoshinori [Auteur]
RIKEN Center for Sustainable Resource Science [Yokohama] [RIKEN CSRS]
Cenci, Ugo [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Colleoni, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Ball, Steven [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Tsuzuki, Mikio [Auteur]
Tokyo University of Pharmacy and Life Sciences [TUPLS]
Fujiwara, Shoko [Auteur]
Tokyo University of Pharmacy and Life Sciences [TUPLS]

Titre de la revue :

Frontiers in Plant Science

Nom court de la revue :

Front. Plant Sci.

Numéro :

13

Pagination :

967165

Éditeur :

Frontiers Media SA

Date de publication :

2022-08-16

ISSN :

1664-462X

Mot(s)-clé(s) en anglais :

Cyanidioschyzon
floridean starch
isoamylase
red algae
semi-amylopectin

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Most rhodophytes synthesize semi-amylopectin as a storage polysaccharide, whereas some species in the most primitive class (Cyanidiophyceae) make glycogen. To know the roles of isoamylases in semi-amylopectin synthesis, ...
Lire la suite >Most rhodophytes synthesize semi-amylopectin as a storage polysaccharide, whereas some species in the most primitive class (Cyanidiophyceae) make glycogen. To know the roles of isoamylases in semi-amylopectin synthesis, we investigated the effects of isoamylase gene (CMI294C and CMS197C)-deficiencies on semi-amylopectin molecular structure and starch granule morphology in Cyanidioschyzon merolae (Cyanidiophyceae). Semi-amylopectin content in a CMS197C-disruption mutant (ΔCMS197C) was not significantly different from that in the control strain, while that in a CMI294C-disruption mutant (ΔCMI294C) was much lower than those in the control strain, suggesting that CMI294C is essential for semi-amylopectin synthesis. Scanning electron microscopy showed that the ΔCMI294C strain contained smaller starch granules, while the ΔCMS197C strain had normal size, but donut-shaped granules, unlike those of the control strain. Although the chain length distribution of starch from the control strain displayed a semi-amylopectin pattern with a peak around degree of polymerization (DP) 11–13, differences in chain length profiles revealed that the ΔCMS197C strain has more short chains (DP of 3 and 4) than the control strain, while the ΔCMI294C strain has more long chains (DP ≥12). These findings suggest that CMI294C-type isoamylase, which can debranch a wide range of chains, probably plays an important role in semi-amylopectin synthesis unique in the Rhodophyta.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

La biochimie comparative et la convergence évolutive : de nouveaux outils de recherche sur la structure et la fonction des voies métaboliques

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Génétique microbienne

Date de dépôt :

2022-09-20T13:45:58Z
2022-09-21T13:21:08Z