Structure-Function analysis of Lactiplantibacillus ...
Document type :
Pré-publication ou Document de travail
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Title :
Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth
Author(s) :
Nikolopoulos, Nikos [Auteur]
Matos, Renata [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Ravaud, Stephanie [Auteur]
Courtin, Pascal [Auteur]
Akherraz, Houssam [Auteur]
Palussiere, Simon [Auteur]
Gueguen-Chaignon, Virginie [Auteur]
Salomon-Mallet, Marie [Auteur]
Guillot, Alain [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
307656|||Gifu University
Chapot-Chartier, Marie-Pierre [Auteur]
Grangeasse, Christophe [Auteur]
Leulier, Francois [Auteur]
Matos, Renata [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Ravaud, Stephanie [Auteur]
Courtin, Pascal [Auteur]
Akherraz, Houssam [Auteur]
Palussiere, Simon [Auteur]
Gueguen-Chaignon, Virginie [Auteur]
Salomon-Mallet, Marie [Auteur]
Guillot, Alain [Auteur]
Guerardel, Yann [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
307656|||Gifu University
Chapot-Chartier, Marie-Pierre [Auteur]
Grangeasse, Christophe [Auteur]
Leulier, Francois [Auteur]
Production date :
2022-09-14
HAL domain(s) :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial ...
Show more >Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that LTAs but not WTAs are D-alanylated in Lactiplantibacillus plantarum NC8 cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum NC8 and Drosophila melanogaster as a symbiosis model, we establish that D-Ala-LTAs are direct symbiotic cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTA to host physiology in other symbiotic models.Show less >
Show more >Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that LTAs but not WTAs are D-alanylated in Lactiplantibacillus plantarum NC8 cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum NC8 and Drosophila melanogaster as a symbiosis model, we establish that D-Ala-LTAs are direct symbiotic cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTA to host physiology in other symbiotic models.Show less >
Language :
Anglais
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Chemical Glycobiology
Submission date :
2022-11-16T11:10:41Z
2022-11-18T11:09:07Z
2022-11-18T11:09:07Z
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