Titre :

Bioinformatics modelling and metabolic engineering of thebranched chain amino acid pathway for specific production of mycosubtilin isoforms in Bacillus subtilis

Auteur(s) :

Guez, Jean Sébastien [Auteur]
Institut national polytechnique Clermont Auvergne [INP Clermont Auvergne]
Coucheney, Francoise [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Castéra-Guy, Joany [Auteur]
Bechet, Max [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Fontanille, Pierre [Auteur]
Chihib, Nour-Eddine [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Niehren, Joachim [Auteur]
BioComputing
Linking Dynamic Data [LINKS]
Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189
Coutte, François [Auteur]
Jacques, Philippe [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158

Titre de la revue :

Metabolites

Éditeur :

MDPI

Date de publication :

2022-01-19

ISSN :

2218-1989

Mot(s)-clé(s) en anglais :

metabolic engineering
bioinformatic modelling
lipopeptides
mycosubtilin
antifungal
fatty acids
branched chain amino acids
Bacillus subtilis

Discipline(s) HAL :

Informatique [cs]/Bio-informatique [q-bio.QM]
Sciences du Vivant [q-bio]/Biotechnologies

Résumé en anglais : [en]

Mycosubtilin belongs to the family of lipopeptides. Different isoforms with various antifungal activities can be obtained according to the length and the isomery of the fatty acid. In this work, the activities of the ...
Lire la suite >Mycosubtilin belongs to the family of lipopeptides. Different isoforms with various antifungal activities can be obtained according to the length and the isomery of the fatty acid. In this work, the activities of the mycosubtilin isoforms were first studied against the pathogen Aspergillus niger, revealing the high activity of the anteiso-C17 isoform. Modification of the mycosubtilin isoform patterns during cultures of the natural strain Bacillus subtilis ATCC 6633 was then investigated through amino acids feeding experiments. In parallel, single gene knockouts and single gene overexpression leading to the overproduction of the anteiso-C15 fatty acid chains were predicted using informatics tools which provide logical reasoning with formal models of reaction networks. In this way, it was in silico predicted that the single overexpression of the ilvA gene as well as the single knockout of the codY gene may lead to the overproduction of anteiso-C15 fatty acid chains. For the first time, it has been demonstrated that overexpression of ilvA allows an enhancement of furniture of odd anteiso fatty acids leading to a favoured mycosubtilin anteiso-C17 production pattern (+41%). Alternatively, a knock-out codY mutant led to a higher furniture of even iso fatty acids, leading to a favoured mycosubtilin iso-C16 production pattern (+180%). These results showed that increased selective synthesis of particular isoforms of mycosubtilin through metabolic engineering is feasible, disclosing the interest of these approaches for future development of lipopeptide producing strains.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS
INRAE
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Équipe(s) de recherche :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Date de dépôt :

2022-12-01T08:51:51Z
2022-12-01T08:53:05Z