Title :

Evaluation of the Efficacy of Dexamethasone-Eluting Electrode Array on the Post-Implant Cochlear Fibrotic Reaction by Three-Dimensional Immunofluorescence Analysis in Mongolian Gerbil Cochlea

Author(s) :

Toulemonde, Philippine [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Risoud, Mickaël [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Lemesre, Pierre-Emmanuel [Auteur]
Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS]
Beck, Cyril [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Wattelet, Jean [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Tardivel, Meryem [Auteur]
Faculté de Médecine Henri Warembourg - Université de Lille
Siepmann, Juergen [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008
Vincent, Christophe [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008

Journal title :

Journal of Clinical Forensic Medicine

Abbreviated title :

J Clin Med

Volume number :

10

Pages :

3315

Publisher :

MDPI

Publication date :

2021-08-17

ISSN :

2077-0383

Keyword(s) :

auditory implant
dexamethasone
hearing loss
rehabilitation
clearing
imaging
light-sheet microscopy

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Cochlear implant is the method of choice for the rehabilitation of severe to profound sensorineural hearing loss. The study of the tissue response to cochlear implantation and the prevention of post-cochlear-implant damages ...
Show more >Cochlear implant is the method of choice for the rehabilitation of severe to profound sensorineural hearing loss. The study of the tissue response to cochlear implantation and the prevention of post-cochlear-implant damages are areas of interest in hearing protection research. The objective was to assess the efficacy of dexamethasone-eluting electrode array on endo canal fibrosis formation by three-dimensional immunofluorescence analysis in implanted Mongolian gerbil cochlea. Two trials were conducted after surgery using Mongolian gerbil implanted with dexamethasone-eluting or non-eluting intracochlear electrode arrays. The animals were then euthanised 10 weeks after implantation. The cochleae were prepared (electrode array in place) according to a 29-day protocol with immunofluorescent labelling and tissue clearing. The acquisition was carried out using light-sheet microscopy. Imaris software was then used for three-dimensional analysis of the cochleae and quantification of the fibrotic volume. The analysis of 12 cochleae showed a significantly different mean volume of fibrosis (2.16 × 108 μm3 ± 0.15 in the dexamethasone eluting group versus 3.17 × 108 μm3 ± 0.54 in the non-eluting group) (p = 0.004). The cochlear implant used as a corticosteroid delivery system appears to be an encouraging device for the protection of the inner ear against fibrosis induced by implantation. Three-dimensional analysis of the cochlea by light-sheet microscopy was suitable for studying post-implantation tissue damage.Show less >

Language :

Anglais

Audience :

Internationale

Administrative institution(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Advanced Drug Delivery Systems (ADDS) - U1008

Submission date :

2022-12-08T01:23:56Z
2023-02-22T10:12:04Z
2023-02-22T11:57:53Z