Title :

Mechanism of competence activation by the ComRS signalling system in streptococci

Author(s) :

Fontaine, Laetitia [Auteur]
Goffin, Philippe [Auteur]
Dubout, Helene [Auteur]
Delplace, Brigitte [Auteur]
Baulard, Alain [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lecat-Guillet, Nathalie [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Chambellon, Emilie [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Gardan, Rozenn [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Hols, Pascal [Auteur]

Journal title :

Molecular Microbiology

Pages :

1113 - 1132

Publisher :

Wiley

Publication date :

2013

ISSN :

0950-382X

English keyword(s) :

NATURAL GENETIC-TRANSFORMATION
GRAM-POSITIVE BACTERIA
CHEMICALLY-DEFINED MEDIUM
BACILLUS-CEREUS GROUP
TRANSCRIPTION INITIATION
GENUS STREPTOCOCCUS
BIOACTIVE PEPTIDES
MILK-PROTEINS
THERMOPHILUS
PNEUMONIAE

HAL domain(s) :

Sciences du Vivant [q-bio]/Sciences agricoles

English abstract : [en]

In many streptococci, competence for natural DNA transformation is regulated by the Rgg-type regulator ComR and the pheromone ComS, which is sensed intracellularly. We compared the ComRS systems of four model streptococcal ...
Show more >In many streptococci, competence for natural DNA transformation is regulated by the Rgg-type regulator ComR and the pheromone ComS, which is sensed intracellularly. We compared the ComRS systems of four model streptococcal species using in vitro and in silico approaches, to determine the mechanism of the ComRS-dependent regulation of competence. In all systems investigated, ComR was shown to be the proximal transcriptional activator of the expression of key competence genes. Efficient binding of ComR to DNA is strictly dependent on the presence of the pheromone (C-terminal ComS octapeptide), in contrast with other streptococcal Rgg-type regulators. The 20bp palindromic ComR-box is the minimal genetic requirement for binding of ComR, and its sequence directly determines the expression level of genes under its control. Despite the apparent species-specific specialization of the ComRComS interaction, mutagenesis of ComS residues from Streptococcus thermophilus highlighted an unexpected permissiveness with respect to its biological activity. In agreement, heterologous ComS, and even primary sequence-unrelated, casein-derived octapeptides, were able to induce competence development in S.thermophilus. The lack of stringency of ComS sequence suggests that competence of a specific Streptococcus species may be modulated by other streptococci or by non-specific nutritive oligopeptides present in its environment.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Non spécifiée

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

Submission date :

2023-05-07T06:59:21Z