Titre :

Novel opto-fluidic drug delivery system for efficient cellular transfection

Auteur(s) :

Layachi, Majid []
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Laboratoire Charles Coulomb [L2C]
Treizebre, Anthony [Auteur]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Hay, Laurent [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Gilbert, David [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Pesez, Jean [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
D’acremont, Quentin [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Braeckmans, Kevin [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Thommen, Quentin [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Courtade, Emmanuel [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]

Titre de la revue :

Journal of Nanobiotechnology

Pagination :

43

Éditeur :

BioMed Central

Date de publication :

2023

ISSN :

1477-3155

Mot(s)-clé(s) en anglais :

Photoporation
Vapour nanobubbles
Microfuidics
High-throughput intracellular delivery
Nanoparticle micro-positioning

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Sciences pharmaceutiques
Sciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique

Résumé en anglais : [en]

Abstract Intracellular drug delivery is at the heart of many diagnosis procedures and a key step in gene therapy. Research has been conducted to bypass cell barriers for controlled intracellular drug release and made ...
Lire la suite >Abstract Intracellular drug delivery is at the heart of many diagnosis procedures and a key step in gene therapy. Research has been conducted to bypass cell barriers for controlled intracellular drug release and made consistent progress. However, state-of-the-art techniques based on non-viral carriers or physical methods suffer several drawbacks, including limited delivery yield, low throughput or low viability, which are key parameters in therapeutics, diagnostics and drug delivery. Nevertheless, gold nanoparticle (AuNP) mediated photoporation has stood out as a promising approach to permeabilize cell membranes through laser induced Vapour NanoBubble (VNB) generation, allowing the influx of external cargo molecules into cells. However, its use as a transfection technology for the genetic manipulation of therapeutic cells is hindered by the presence of non-degradable gold nanoparticles. Here, we report a new optofluidic method bringing gold nanoparticles in close proximity to cells for photoporation, while avoiding direct contact with cells by taking advantage of hydrodynamic focusing in a multi-flow device. Cells were successfully photoporated with $$\sim {70}{\%}$$ ∼ 70 % efficiency with no significant reduction in cell viability at a throughput ranging from $$10^3$$ 10 3 to $$10^4~\text {cells}~{\hbox {min}^{-1}}$$ 10 4 cells min - 1 . This optofluidic approach provides prospects of translating photoporation from an R &D setting to clinical use for producing genetically engineered therapeutic cells.Lire moins >

Langue :

Anglais

Vulgarisation :

Non

Projet ANR :

Centre Européen pour les Mathématiques, la Physique et leurs Interactions

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL