Title :

Wild Wheat Rhizosphere-Associated Plant Growth-Promoting Bacteria Exudates: Effect on Root Development in Modern Wheat and Composition

Author(s) :

Zhour, Houssein [Auteur]
Institut des Sciences des Plantes de Montpellier [IPSIM]
Bray, Fabrice [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Dandache, Israa [Auteur]
Marti, Guillaume [Auteur]
MetaToul Agromix
Flament, Stéphanie [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Perez, Amélie [Auteur]
MetaToul Agromix
Lis, Maëlle [Auteur]
Écophysiologie des Plantes sous Stress environnementaux [LEPSE]
Cabrera-Bosquet, Llorenç [Auteur]
Écophysiologie des Plantes sous Stress environnementaux [LEPSE]
Perez, Thibaut [Auteur]
Institut des Sciences des Plantes de Montpellier [IPSIM]
Fizames, Cécile [Auteur]
Institut des Sciences des Plantes de Montpellier [IPSIM]
Baudoin, Ezekiel [Auteur]
Laboratoire des symbioses tropicales et méditerranéennes [UMR LSTM]
Madani, Ikram [Auteur]
Institut des Sciences des Plantes de Montpellier [IPSIM]
El Zein, Loubna [Auteur]
Véry, Anne-Aliénor [Auteur]
Institut des Sciences des Plantes de Montpellier [IPSIM]
Rolando, Christian [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Sentenac, Hervé [Auteur]
Institut des Sciences des Plantes de Montpellier [IPSIM]
Chokr, Ali [Auteur]
Peltier, Jean-Benoît [Auteur correspondant]
Institut des Sciences des Plantes de Montpellier [IPSIM]

Journal title :

International Journal of Molecular Sciences

Pages :

15248

Publisher :

MDPI

Publication date :

2022

ISSN :

1661-6596

English keyword(s) :

diazotroph
durum wheat
metabolomics
plant–microbiome communication
proteomics
PGPR
bacterial exudate

HAL domain(s) :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
Sciences du Vivant [q-bio]/Biologie végétale/Botanique
Sciences du Vivant [q-bio]/Ecologie, Environnement/Interactions entre organismes

English abstract : [en]

Diazotrophic bacteria isolated from the rhizosphere of a wild wheat ancestor, grown from its refuge area in the Fertile Crescent, were found to be efficient Plant Growth-Promoting Rhizobacteria (PGPR), upon interaction ...
Show more >Diazotrophic bacteria isolated from the rhizosphere of a wild wheat ancestor, grown from its refuge area in the Fertile Crescent, were found to be efficient Plant Growth-Promoting Rhizobacteria (PGPR), upon interaction with an elite wheat cultivar. In nitrogen-starved plants, they increased the amount of nitrogen in the seed crop (per plant) by about twofold. A bacterial growth medium was developed to investigate the effects of bacterial exudates on root development in the elite cultivar, and to analyze the exo-metabolomes and exo-proteomes. Altered root development was observed, with distinct responses depending on the strain, for instance, with respect to root hair development. A first conclusion from these results is that the ability of wheat to establish effective beneficial interactions with PGPRs does not appear to have undergone systematic deep reprogramming during domestication. Exo-metabolome analysis revealed a complex set of secondary metabolites, including nutrient ion chelators, cyclopeptides that could act as phytohormone mimetics, and quorum sensing molecules having inter-kingdom signaling properties. The exo-proteome-comprised strain-specific enzymes, and structural proteins belonging to outer-membrane vesicles, are likely to sequester metabolites in their lumen. Thus, the methodological processes we have developed to collect and analyze bacterial exudates have revealed that PGPRs constitutively exude a highly complex set of metabolites; this is likely to allow numerous mechanisms to simultaneously contribute to plant growth promotion, and thereby to also broaden the spectra of plant genotypes (species and accessions/cultivars) with which beneficial interactions can occur.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290

Source :

Harvested from HAL