The PPARγ-dependent effect of flavonoid ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
The PPARγ-dependent effect of flavonoid luteolin against damage induced by the chemotherapeutic irinotecan in human intestinal cells.
Author(s) :
Boeing, Thaise [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Speca, Silvia [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
De Souza, Priscila [Auteur]
Universidade do Vale do Itajaí [UNIVALI]
Martin Mena, Anthony [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Bertin, Benjamin [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Desreumax, Pierre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Mota Da Silva, Luis [Auteur]
Universidade do Vale do Itajaí [UNIVALI]
Faloni De Andrade, Sergio [Auteur]
Universidade do Vale do Itajaí [UNIVALI]
Dubuqoy, Laurent [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]
Speca, Silvia [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
De Souza, Priscila [Auteur]
Universidade do Vale do Itajaí [UNIVALI]
Martin Mena, Anthony [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Bertin, Benjamin [Auteur]

Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Desreumax, Pierre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Mota Da Silva, Luis [Auteur]
Universidade do Vale do Itajaí [UNIVALI]
Faloni De Andrade, Sergio [Auteur]
Universidade do Vale do Itajaí [UNIVALI]
Dubuqoy, Laurent [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Journal title :
Chemico-Biological Interactions
Abbreviated title :
Chem Biol Interact
Volume number :
351
Pages :
109712
Publisher :
Elsevier
Publication date :
2021-10-28
ISSN :
1872-7786
Keyword(s) :
Rosiglitazone
Chemotherapy
Oxidative stress
Inflammation
Caco-2
Intestinal mucositis
Chemotherapy
Oxidative stress
Inflammation
Caco-2
Intestinal mucositis
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Irinotecan (CPT-11) is one of the main agents used to treat colorectal cancer; unfortunately, it is associated with increased intestinal mucositis developing. Luteolin has been shown to prevent damage induced by this ...
Show more >Irinotecan (CPT-11) is one of the main agents used to treat colorectal cancer; unfortunately, it is associated with increased intestinal mucositis developing. Luteolin has been shown to prevent damage induced by this chemotherapeutic in mice; thus, in this research, we have investigated luteolin's action mechanism in human intestinal epithelial cells. The potential of luteolin in reducing inflammation and oxidative stress induced by irinotecan in Caco-2 cells was evaluated by PCR through mRNA expression of inflammatory and oxidative genes and by ELISA at the protein level. To assess whether luteolin's ability to control irinotecan-induced damage occurs in a PPARγ dependent manner, experiments were performed on PPARγ downregulated cells. Irinotecan downregulated PPARγ expression and upregulated inflammatory and oxidative genes, while luteolin upregulated PPARγ, HO-1, SOD and decreased expression of IL-1β and iNOS. Interestingly, when the cells were co-stimulated with luteolin and irinotecan, the flavonoid reversed the inflammation and oxidative imbalance evoked by the chemotherapeutic. However, when these experiments were performed in cells downregulated for PPARγ, luteolin lost the capacity to increase PPARγ and reverse the effect of irinotecan in all tested genes, except by IL-1β. The present study showed that the protective effect of luteolin against irinotecan is PPARγ dependent.Show less >
Show more >Irinotecan (CPT-11) is one of the main agents used to treat colorectal cancer; unfortunately, it is associated with increased intestinal mucositis developing. Luteolin has been shown to prevent damage induced by this chemotherapeutic in mice; thus, in this research, we have investigated luteolin's action mechanism in human intestinal epithelial cells. The potential of luteolin in reducing inflammation and oxidative stress induced by irinotecan in Caco-2 cells was evaluated by PCR through mRNA expression of inflammatory and oxidative genes and by ELISA at the protein level. To assess whether luteolin's ability to control irinotecan-induced damage occurs in a PPARγ dependent manner, experiments were performed on PPARγ downregulated cells. Irinotecan downregulated PPARγ expression and upregulated inflammatory and oxidative genes, while luteolin upregulated PPARγ, HO-1, SOD and decreased expression of IL-1β and iNOS. Interestingly, when the cells were co-stimulated with luteolin and irinotecan, the flavonoid reversed the inflammation and oxidative imbalance evoked by the chemotherapeutic. However, when these experiments were performed in cells downregulated for PPARγ, luteolin lost the capacity to increase PPARγ and reverse the effect of irinotecan in all tested genes, except by IL-1β. The present study showed that the protective effect of luteolin against irinotecan is PPARγ dependent.Show less >
Language :
Anglais
Audience :
Internationale
Administrative institution(s) :
Université de Lille
CHU Lille
CHU Lille
Collections :
Submission date :
2023-05-25T02:56:38Z
2023-08-23T07:15:09Z
2023-08-23T07:15:09Z