Electroseparation of Slaughterhouse ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification.
Author(s) :
Przybylski, Rémi [Auteur]
Institut Sophia Agrobiotech [ISA]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Bazinet, Laurent [Auteur]
Institut sur la Nutrition et les Aliments Fonctionnels [université Laval, Québec] [INAF]
Firdaous, Loubna [Auteur]
Institut Sophia Agrobiotech [ISA]
Kouach, Mostafa [Auteur]
Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Goossens, Jean-Francois [Auteur]
Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Dhulster, Pascal [Auteur]
Institut Sophia Agrobiotech [ISA]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Nedjar-Arroume, Naïma [Auteur]
Institut Sophia Agrobiotech [ISA]
Institut Charles Viollette (ICV) - ULR 7394 [ICV]
Institut Sophia Agrobiotech [ISA]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Bazinet, Laurent [Auteur]
Institut sur la Nutrition et les Aliments Fonctionnels [université Laval, Québec] [INAF]
Firdaous, Loubna [Auteur]
Institut Sophia Agrobiotech [ISA]
Kouach, Mostafa [Auteur]

Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Goossens, Jean-Francois [Auteur]

Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Dhulster, Pascal [Auteur]

Institut Sophia Agrobiotech [ISA]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Nedjar-Arroume, Naïma [Auteur]
Institut Sophia Agrobiotech [ISA]
Institut Charles Viollette (ICV) - ULR 7394 [ICV]
Journal title :
Membranes (Basel)
Volume number :
10
Pages :
90
Publisher :
MDPI
Publication date :
2020-05-09
ISSN :
2077-0375
Keyword(s) :
waste valorisation
antimicrobial peptide
ultrafiltration membrane
electrodialysis
antimicrobial peptide
ultrafiltration membrane
electrodialysis
HAL domain(s) :
Sciences du Vivant [q-bio]
French abstract :
The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to ...
Show more >The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137–141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the α137–141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase—75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the α137–141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF α137–141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.Show less >
Show more >The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137–141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the α137–141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase—75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the α137–141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF α137–141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.Show less >
Language :
Anglais
Audience :
Internationale
Administrative institution(s) :
Université de Lille
CHU Lille
CHU Lille
Collections :
Submission date :
2023-05-25T03:34:57Z
2023-09-20T07:40:38Z
2023-09-20T07:40:38Z
Files
- membranes-10-00090-v2.pdf
- Non spécifié
- Open access
- Access the document