Nano-Structured Ridged Micro-Filaments ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Nano-Structured Ridged Micro-Filaments (≥100 µm Diameter) Produced Using a Single Step Strategy for Improved Bone Cell Adhesion and Proliferation in Textile Scaffolds.
Auteur(s) :
Behary, N. [Auteur]
Génie des Matériaux Textiles - ULR 2461 [GEMTEX]
Eap, S. [Auteur]
Cayla, A. [Auteur]
Génie des Matériaux Textiles - ULR 2461 [GEMTEX]
Hildebrand, Feng [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008
Benkirane-Jessel, N. [Auteur]
Campagne, C. [Auteur]
Génie et Matériaux Textiles [GEMTEX]
Génie des Matériaux Textiles - ULR 2461 [GEMTEX]
Eap, S. [Auteur]
Cayla, A. [Auteur]
Génie des Matériaux Textiles - ULR 2461 [GEMTEX]
Hildebrand, Feng [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008
Benkirane-Jessel, N. [Auteur]
Campagne, C. [Auteur]
Génie et Matériaux Textiles [GEMTEX]
Titre de la revue :
Molecules
Numéro :
27
Date de publication :
2022-07-01
ISSN :
1420-3049
Mot(s)-clé(s) :
microfilaments
PLLA
nano-ridged fiber surface
melt-spinning
bone cell engineering
osteogenic expression
PLLA
nano-ridged fiber surface
melt-spinning
bone cell engineering
osteogenic expression
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Textile scaffolds that are either 2D or 3D with tunable shapes and pore sizes can be made through textile processing (weaving, knitting, braiding, nonwovens) using microfilaments. However, these filaments lack nano-topographical ...
Lire la suite >Textile scaffolds that are either 2D or 3D with tunable shapes and pore sizes can be made through textile processing (weaving, knitting, braiding, nonwovens) using microfilaments. However, these filaments lack nano-topographical features to improve bone cell adhesion and proliferation. Moreover, the diameter of such filaments should be higher than that used for classical textiles (10−30 µm) to enable adhesion and the efficient spreading of the osteoblast cell (>30 µm diameter). We report, for the first time, the fabrication of biodegradable nanostructured cylindrical PLLA (poly-L-Lactic acid) microfilaments of diameters 100 µm and 230 µm, using a single step melt-spinning process for straightforward integration of nano-scale ridge-like structures oriented in the fiber length direction. Appropriate drawing speed and temperature used during the filament spinning allowed for the creation of instabilities giving rise to nanofibrillar ridges, as observed by AFM (Atomic Force Microscopy). These micro-filaments were hydrophobic, and had reduced crystallinity and mechanical strength, but could still be processed into 2D/3D textile scaffolds of various shapes. Biological tests carried out on the woven scaffolds made from these nano-structured micro filaments showed excellent human bone cell MG 63 adhesion and proliferation, better than on smooth 30 µm- diameter fibers. Elongated filopodia of the osteoblast, intimately anchored to the nano-structured filaments, was observed. The filaments also induced in vitro osteogenic expression, as shown by the expression of osteocalcin and bone sialoprotein after 21 days of culture. This work deals with the fabrication of a new generation of nano-structured micro-filament for use as scaffolds of different shapes suited for bone cell engineering.Lire moins >
Lire la suite >Textile scaffolds that are either 2D or 3D with tunable shapes and pore sizes can be made through textile processing (weaving, knitting, braiding, nonwovens) using microfilaments. However, these filaments lack nano-topographical features to improve bone cell adhesion and proliferation. Moreover, the diameter of such filaments should be higher than that used for classical textiles (10−30 µm) to enable adhesion and the efficient spreading of the osteoblast cell (>30 µm diameter). We report, for the first time, the fabrication of biodegradable nanostructured cylindrical PLLA (poly-L-Lactic acid) microfilaments of diameters 100 µm and 230 µm, using a single step melt-spinning process for straightforward integration of nano-scale ridge-like structures oriented in the fiber length direction. Appropriate drawing speed and temperature used during the filament spinning allowed for the creation of instabilities giving rise to nanofibrillar ridges, as observed by AFM (Atomic Force Microscopy). These micro-filaments were hydrophobic, and had reduced crystallinity and mechanical strength, but could still be processed into 2D/3D textile scaffolds of various shapes. Biological tests carried out on the woven scaffolds made from these nano-structured micro filaments showed excellent human bone cell MG 63 adhesion and proliferation, better than on smooth 30 µm- diameter fibers. Elongated filopodia of the osteoblast, intimately anchored to the nano-structured filaments, was observed. The filaments also induced in vitro osteogenic expression, as shown by the expression of osteocalcin and bone sialoprotein after 21 days of culture. This work deals with the fabrication of a new generation of nano-structured micro-filament for use as scaffolds of different shapes suited for bone cell engineering.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Date de dépôt :
2023-05-30T06:52:23Z
2023-08-30T08:12:01Z
2023-08-30T08:12:01Z