Titre :

Protein-mediated dethreading of a biotin-functionalised pseudorotaxane

Auteur(s) :

Caldwell, Stuart T [Auteur]
University of Glasgow
Maclean, Catherine [Auteur]
University of Glasgow
Riehle, Mathis [Auteur]
University of Glasgow
Cooper, Alan [Auteur]
University of Glasgow
Nutley, Margaret [Auteur]
University of Glasgow
Rabani, Gouher [Auteur]
University of Glasgow
Fitzpatrick, Brian [Auteur]
University of Glasgow
Rotello, Vincent [Auteur]
Department of Chemistry [Amherst]
O Smith, Brian [Auteur]
University of Glasgow
Belal, Khaled [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Woisel, Patrice [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Cooke, Graeme [Auteur]
University of Glasgow

Titre de la revue :

Organic & Biomolecular Chemistry

Numéro :

12

Pagination :

511-516

Date de publication :

2014

Discipline(s) HAL :

Sciences de l'ingénieur [physics]/Matériaux
Sciences de l'ingénieur [physics]/Génie des procédés
Chimie/Matériaux
Chimie/Polymères

Résumé en anglais : [en]

In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques. We have shown ...
Lire la suite >In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques. We have shown using 2-(4′-hydroxyazobenzene)benzoic acid displacement and ITC experiments, that compounds 3 and 4 have the propensity to form reasonably high-affinity bioconjugates with avidin and neutravidin. We have also demonstrated using 1H NMR, UV-vis and fluorescence spectroscopy that the naphthalene moiety of 3 and 4 facilitates the formation of pseudorotaxane-like structures with 1 in water. We have then investigated the ability of avidin and neutravidin to modulate the complexation between 1 and 3 or 4. UV-vis and fluorescence spectroscopy has shown that in both cases the addition of the protein disrupts complexation between the naphthalene moieties of 3 and 4 with 1.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
ENSCL
CNRS
INRA

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Équipe(s) de recherche :

Ingénierie des Systèmes Polymères

Date de dépôt :

2019-05-16T16:01:06Z
2020-04-16T13:03:57Z