Screening of Antimicrobial Activities and ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Screening of Antimicrobial Activities and Lipopeptide Production of Endophytic Bacteria Isolated from Vetiver Roots
Author(s) :
Munakata, Yuka [Auteur]
Heuson, Egon [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181
Daboudet, Theo [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Deracinois, Barbara [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Duban, Matthieu [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Hehn, Alain [Auteur]
Coutte, Francois [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Slezack-Deschaumes, Sophie [Auteur]
Heuson, Egon [Auteur]
Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181
Daboudet, Theo [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Deracinois, Barbara [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Duban, Matthieu [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Hehn, Alain [Auteur]
Coutte, Francois [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut Charles Viollette (ICV) - ULR 7394
Slezack-Deschaumes, Sophie [Auteur]
Journal title :
Microorganisms
Volume number :
10
Pages :
209
Publisher :
MDPI
Publication date :
2022-01-19
ISSN :
2076-2607
English keyword(s) :
Fusarium sp
Bacillus subtilis
high-throughput screening
antifungal activities
lipopeptides
bacterial endophytes
Bacillus subtilis
high-throughput screening
antifungal activities
lipopeptides
bacterial endophytes
HAL domain(s) :
Sciences du Vivant [q-bio]
Chimie/Catalyse
Chimie/Catalyse
English abstract : [en]
The exploration of certain microbial resources such as beneficial endophytic microorganisms is considered a promising strategy for the discovery of new antimicrobial compounds for the pharmaceutical industries and agriculture. ...
Show more >The exploration of certain microbial resources such as beneficial endophytic microorganisms is considered a promising strategy for the discovery of new antimicrobial compounds for the pharmaceutical industries and agriculture. Thirty-one endophytic bacterial strains affiliated with Bacillus, Janthinobacterium, Yokenella, Enterobacter, Pseudomonas, Serratia, and Microbacterium were previously isolated from vetiver (Chrysopogon zizanioides (L.) Roberty) roots. These endophytes showed antifungal activity against Fusarium graminearum and could be a source of antimicrobial metabolites. In this study, in particular, using high-throughput screening, we analyzed their antagonistic activities and those of their cell-free culture supernatants against three species of Fusarium plant pathogens, a bacterial strain of Escherichia coli, and a yeast strain of Saccharomyces cerevisiae, as well as their capacity to produce lipopeptides. The results showed that the culture supernatants of four strains close to B. subtilis species exhibited antimicrobial activities against Fusarium species and E. coli. Using mass spectrometry analyses, we identified two groups of lipopeptides (surfactins and plipastatins) in their culture supernatants. Whole-genome sequencing confirmed that these bacteria possess NRPS gene clusters for surfactin and plipastatin. In vitro tests confirmed the inhibitory effect of plipastatin alone or in combination with surfactin against the three Fusarium species.Show less >
Show more >The exploration of certain microbial resources such as beneficial endophytic microorganisms is considered a promising strategy for the discovery of new antimicrobial compounds for the pharmaceutical industries and agriculture. Thirty-one endophytic bacterial strains affiliated with Bacillus, Janthinobacterium, Yokenella, Enterobacter, Pseudomonas, Serratia, and Microbacterium were previously isolated from vetiver (Chrysopogon zizanioides (L.) Roberty) roots. These endophytes showed antifungal activity against Fusarium graminearum and could be a source of antimicrobial metabolites. In this study, in particular, using high-throughput screening, we analyzed their antagonistic activities and those of their cell-free culture supernatants against three species of Fusarium plant pathogens, a bacterial strain of Escherichia coli, and a yeast strain of Saccharomyces cerevisiae, as well as their capacity to produce lipopeptides. The results showed that the culture supernatants of four strains close to B. subtilis species exhibited antimicrobial activities against Fusarium species and E. coli. Using mass spectrometry analyses, we identified two groups of lipopeptides (surfactins and plipastatins) in their culture supernatants. Whole-genome sequencing confirmed that these bacteria possess NRPS gene clusters for surfactin and plipastatin. In vitro tests confirmed the inhibitory effect of plipastatin alone or in combination with surfactin against the three Fusarium species.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
ANR Project :
Administrative institution(s) :
Université de Lille
CNRS
Centrale Lille
ENSCL
Univ. Artois
CNRS
Centrale Lille
ENSCL
Univ. Artois
Research team(s) :
Valorisation des alcanes et de la biomasse (VAALBIO)
Submission date :
2023-05-30T15:21:19Z
2023-06-21T10:06:36Z
2023-06-21T10:06:36Z
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