Role Played by Exosporium Glycoproteins ...
Type de document :
Article dans une revue scientifique
DOI :
URL permanente :
Titre :
Role Played by Exosporium Glycoproteins in the Surface Properties of Bacillus cereus Spores and in Their Adhesion to Stainless Steel
Auteur(s) :
Lequette, Yannick [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Garenaux, Estelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Tauveron, Grégoire [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Dumez, Sylvain [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Perchat, Stéphane [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Slomianny, Christian [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Lereclus, Didier [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Garenaux, Estelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Tauveron, Grégoire [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Dumez, Sylvain [Auteur]

Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Perchat, Stéphane [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Slomianny, Christian [Auteur]

Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Lereclus, Didier [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Guerardel, Yann [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Faille, Christine [Auteur]

Unité Matériaux et Transformations - UMR 8207 [UMET]
Titre de la revue :
Applied and Environmental Microbiology
Numéro :
77
Pagination :
4905-4911
Date de publication :
2011-07
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Ingénierie des aliments
Physique [physics]/Matière Condensée [cond-mat]/Science des matériaux [cond-mat.mtrl-sci]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
Sciences de l'ingénieur [physics]/Génie des procédés
Physique [physics]/Matière Condensée [cond-mat]/Science des matériaux [cond-mat.mtrl-sci]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
Sciences de l'ingénieur [physics]/Génie des procédés
Résumé en anglais : [en]
Bacillus cereus spores are surrounded by a loose-fitting layer called the exosporium, whose distal part is mainly formed from glycoproteins. The role played by the exosporium glycoproteins of B. cereus ATCC 14579 (BclA and ...
Lire la suite >Bacillus cereus spores are surrounded by a loose-fitting layer called the exosporium, whose distal part is mainly formed from glycoproteins. The role played by the exosporium glycoproteins of B. cereus ATCC 14579 (BclA and ExsH) was investigated by considering hydrophobicity and charge, as well as the properties of spore adhesion to stainless steel. The absence of BclA increased both the isoelectric point (IEP) and hydrophobicity of whole spores while simultaneously reducing the interaction between spores and stainless steel. However, neither the hydrophobicity nor the charge associated with BclA could explain the differences in the adhesion properties. Conversely, ExsH, another exosporium glycoprotein, did not play a significant role in spore surface properties. The monosaccharide analysis of B. cereus ATCC 14579 showed different glycosylation patterns on ExsH and BclA. Moreover, two specific glycosyl residues, namely, 2-O-methyl-rhamnose (2-Me-Rha) and 2,4-O-methyl-rhamnose (2,4-Me-Rha), were attached to BclA, in addition to the glycosyl residues already reported in B. anthracis.Lire moins >
Lire la suite >Bacillus cereus spores are surrounded by a loose-fitting layer called the exosporium, whose distal part is mainly formed from glycoproteins. The role played by the exosporium glycoproteins of B. cereus ATCC 14579 (BclA and ExsH) was investigated by considering hydrophobicity and charge, as well as the properties of spore adhesion to stainless steel. The absence of BclA increased both the isoelectric point (IEP) and hydrophobicity of whole spores while simultaneously reducing the interaction between spores and stainless steel. However, neither the hydrophobicity nor the charge associated with BclA could explain the differences in the adhesion properties. Conversely, ExsH, another exosporium glycoprotein, did not play a significant role in spore surface properties. The monosaccharide analysis of B. cereus ATCC 14579 showed different glycosylation patterns on ExsH and BclA. Moreover, two specific glycosyl residues, namely, 2-O-methyl-rhamnose (2-Me-Rha) and 2,4-O-methyl-rhamnose (2,4-Me-Rha), were attached to BclA, in addition to the glycosyl residues already reported in B. anthracis.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
Inserm
CNRS
Inserm
CNRS
Collections :
Équipe(s) de recherche :
Processus aux Interfaces et Hygiène des Matériaux (PIHM)
Date de dépôt :
2019-05-16T16:44:40Z
2021-03-17T07:08:27Z
2021-03-17T07:15:32Z
2021-05-17T13:59:19Z
2021-03-17T07:08:27Z
2021-03-17T07:15:32Z
2021-05-17T13:59:19Z