Titre :

Suppression of the Arabidopsis cinnamoyl-CoA reductase 1-6 intronic T-DNA mutation by epigenetic modification

Auteur(s) :

Bastiaanse, Héloïse [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Vlaams Instituut voor Biotechnologie [Ghent, Belgique] [VIB]
Rombauts, Stephane [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Vlaams Instituut voor Biotechnologie [Ghent, Belgique] [VIB]
de Vries, Lisanne [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Vlaams Instituut voor Biotechnologie [Ghent, Belgique] [VIB]
De Pooter, Tim [Auteur]
University of Antwerp [UA]
Department of Biomedical Sciences [Antwerp]
Strazisar, Mojca [Auteur]
University of Antwerp [UA]
Department of Biomedical Sciences [Antwerp]
Neutelings, Godfrey [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Vanholme, Ruben [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Vlaams Instituut voor Biotechnologie [Ghent, Belgique] [VIB]
Boerjan, Wout [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Vlaams Instituut voor Biotechnologie [Ghent, Belgique] [VIB]

Titre de la revue :

Plant Physiology

Nom court de la revue :

Plant Physiol

Numéro :

192

Pagination :

3001-3016

Éditeur :

Oxford University Press ; American Society of Plant Biologists

Date de publication :

2023-08-04

ISSN :

1532-2548

Mot(s)-clé(s) en anglais :

Arabidopsis
Lignin
Mutation
DNA, Bacterial
Arabidopsis Proteins
Epigenesis, Genetic
Glucosyltransferases

Discipline(s) HAL :

Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

Arabidopsis (Arabidopsis thaliana) transfer DNA (T-DNA) insertion collections are popular resources for fundamental plant research. Cinnamoyl-CoA reductase 1 (CCR1) catalyzes an essential step in the biosynthesis of the ...
Lire la suite >Arabidopsis (Arabidopsis thaliana) transfer DNA (T-DNA) insertion collections are popular resources for fundamental plant research. Cinnamoyl-CoA reductase 1 (CCR1) catalyzes an essential step in the biosynthesis of the cell wall polymer lignin. Accordingly, the intronic T-DNA insertion mutant ccr1-6 has reduced lignin levels and shows a stunted growth phenotype. Here, we report restoration of the ccr1-6 mutant phenotype and CCR1 expression levels after a genetic cross with a UDP-glucosyltransferase 72e1 (ugt72e1),-e2,-e3 T-DNA mutant. We discovered that the phenotypic recovery was not dependent on the UGT72E family loss of function but due to an epigenetic phenomenon called trans T-DNA suppression. Via trans T-DNA suppression, the gene function of an intronic T-DNA mutant was restored after the introduction of an additional T-DNA sharing identical sequences, leading to heterochromatinization and splicing out of the T-DNA-containing intron. Consequently, the suppressed ccr1-6 allele was named epiccr1-6. Long-read sequencing revealed that epiccr1-6, not ccr1-6, carries dense cytosine methylation over the full length of the T-DNA. We showed that the SAIL T-DNA in the UGT72E3 locus could trigger the trans T-DNA suppression of the GABI-Kat T-DNA in the CCR1 locus. Furthermore, we scanned the literature for other potential cases of trans T-DNA suppression in Arabidopsis and found that 22% of the publications matching our query report on double or higher-order T-DNA mutants that meet the minimal requirements for trans T-DNA suppression. These combined observations indicate that intronic T-DNA mutants need to be used with caution since methylation of intronic T-DNA might derepress gene expression and can thereby confound results.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Fibres végétales

Date de dépôt :

2023-08-28T12:14:40Z
2023-09-08T11:34:38Z