Titre :

Activation of mutated TRPA1 ion channel by resveratrol in human prostate cancer associated fibroblasts (CAF)

Auteur(s) :

Vancauwenberghe, Eric [Auteur]
Noyer, Lucile [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Derouiche, Sandra [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lemonnier, Loïc [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Gosset, Pierre [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Sadofsky, Laura [Auteur]
Mariot, Pascal [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Warnier, Marine [Auteur]
Bokhobza, Alexandre [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Slomianny, Christian [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Mauroy, Brigitte [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Bonnal, Jean‐louis [Auteur]
Dewailly, Etienne [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
delcourt, philippe [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Allart, Laurent [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Desruelles, Emilie [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Prevarskaya, Natalia [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Roudbaraki, Morad [Auteur]

Titre de la revue :

Molecular Carcinogenesis

Pagination :

1851-1867

Éditeur :

Wiley

Date de publication :

2017-08

ISSN :

0899-1987

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Previous studies showed the effects of resveratrol (RES) on several cancer cells, including prostate cancer (PCa) cell apoptosis without taking into consideration the impact of the tumor microenvironment (TME). The TME is ...
Lire la suite >Previous studies showed the effects of resveratrol (RES) on several cancer cells, including prostate cancer (PCa) cell apoptosis without taking into consideration the impact of the tumor microenvironment (TME). The TME is composed of cancer cells, endothelial cells, blood cells, and cancer‐associated fibroblasts (CAF), the main source of growth factors. The latter cells might modify in the TME the impact of RES on tumor cells via secreted factors. Recent data clearly show the impact of CAF on cancer cells apoptosis resistance via secreted factors. However, the effects of RES on PCa CAF have not been studied so far. We have investigated here for the first time the effects of RES on the physiology of PCa CAF in the context of TME. Using a prostate cancer CAF cell line and primary cultures of CAF from prostate cancers, we show that RES activates the N‐terminal mutated Transient Receptor Potential Ankyrin 1 (TRPA1) channel leading to an increase in intracellular calcium concentration and the expression and secretion of growth factors (HGF and VEGF) without inducing apoptosis in these cells. Interestingly, in the present work, we also show that when the prostate cancer cells were co‐cultured with CAF, the RES‐induced cancer cell apoptosis was reduced by 40%, an apoptosis reduction canceled in the presence of the TRPA1 channel inhibitors. The present work highlights CAF TRPA1 ion channels as a target for RES and the importance of the channel in the epithelial‐stromal crosstalk in the TME leading to resistance to the RES‐induced apoptosis.Lire moins >

Langue :

Anglais

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL

Date de dépôt :

2023-09-19T04:39:27Z