Title :

Functional coupling between large-conductance potassium channels and Cav3.2 voltage-dependent calcium channels participates in prostate cancer cell growth

Author(s) :

Gackière, Florian [Auteur]
Hôpital de la Timone [CHU - APHM] [TIMONE]
Warnier, Marine [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Katsogiannou, Maria [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Derouiche, Sandra [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
delcourt, philippe [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Dewailly, Etienne [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Slomianny, Christian [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Humez, Sandrine [Auteur]
Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 [JPArc]
Prevarskaya, Natalia [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Roudbaraki, Morad [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Mariot, Pascal [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]

Journal title :

Biology Open

Pages :

941-951

Publisher :

Royal Society

Publication date :

2013-09-15

ISSN :

2046-6390

English keyword(s) :

BK channels
KCa1.1
Cav3.2
CACNA1H
T-type calcium channels
Proliferation
Prostate
Cancer cell growth

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Summary It is strongly suspected that potassium (K+) channels are involved in various aspects of prostate cancer development, such as cell growth. However, the molecular nature of those K+ channels implicated in prostate ...
Show more >Summary It is strongly suspected that potassium (K+) channels are involved in various aspects of prostate cancer development, such as cell growth. However, the molecular nature of those K+ channels implicated in prostate cancer cell proliferation and the mechanisms through which they control proliferation are still unknown. This study uses pharmacological, biophysical and molecular approaches to show that the main voltage-dependent K+ current in prostate cancer LNCaP cells is carried by large-conductance BK channels. Indeed, most of the voltage-dependent current was inhibited by inhibitors of BK channels (paxillin and iberiotoxin) and by siRNA targeting BK channels. In addition, we reveal that BK channels constitute the main K+ channel family involved in setting the resting membrane potential in LNCaP cells at around −40 mV. This consequently promotes a constitutive calcium entry through T-type Cav3.2 calcium channels. We demonstrate, using single-channel recording, confocal imaging and co-immunoprecipitation approaches, that both channels form macromolecular complexes. Finally, using flow cytometry cell cycle measurements, cell survival assays and Ki67 immunofluorescent staining, we show that both BK and Cav3.2 channels participate in the proliferation of prostate cancer cells.Show less >

Language :

Anglais

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL

Submission date :

2023-09-19T04:40:17Z