Title :

Robust Polyion Complex Vesicles (PICsomes) based on PEO‐b‐Poly(Amino Acid) Copolymers Combining Electrostatic and Hydrophobic Interaction: Formation, siRNA Loading and Intracellular Delivery

Author(s) :

Aydinlioglu, Esra [Auteur]
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Abdelghani, Mona [Auteur]
Eindhoven University of Technology [Eindhoven] [TU/e]
Le Fer, Gaëlle [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Unité Matériaux et Transformations (UMET) - UMR 8207
van Hest, Jan C. M. [Auteur]
Eindhoven University of Technology [Eindhoven] [TU/e]
Sandre, Olivier [Auteur correspondant]
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Lecommandoux, Sébastien [Auteur correspondant]
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Journal title :

Macromolecular Chemistry and Physics

Pages :

220306

Publisher :

Wiley-VCH Verlag

Publication date :

2023-01-10

ISSN :

1022-1352

English keyword(s) :

PEO-b-poly(amino acids) block copolymers
PICsomes
siRNAsomes
poly(L-glutamic acid)
poly(L-lysine)
poly(L-phenylalanine)
N-carboxyanhydride (NCA)
ring opening polymerization (ROP)
circular dichroism
small interfering RNA (siRNA)
polyionic complex (PIC) vesicles

HAL domain(s) :

Chimie/Polymères
Sciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacie galénique
Chimie/Matériaux

English abstract : [en]

Two pairs of oppositely charged PEO-b-poly(amino acid) copolymers with neutral poly(ethylene oxide) block and polypeptide block composed of the hydrophobic l-phenylalanine (Phe) amino acid mixed with either negative ...
Show more >Two pairs of oppositely charged PEO-b-poly(amino acid) copolymers with neutral poly(ethylene oxide) block and polypeptide block composed of the hydrophobic l-phenylalanine (Phe) amino acid mixed with either negative l-glutamic acid (Glu) or positive l-lysine (Lys) units were synthesized. N-carboxyanhydride (NCA) ring opening polymerization (ROP) was performed with either PEO46-NH2 or PEO114-NH2 macroinitiators, leading respectively to PEO46-b-P(Glu100-co-Phe65) and PEO46-b-P(Lys100-co-Phe65), and PEO114-b-P(Glu60-co-Phe40) and PEO114-b-P(Lys60-co-Phe40). Polyion complexes (PIC) formed at near charge equilibrium led to vesicle formation (PICsomes), as shown by DLS, zetametry and TEM. The good stability of PICsomes, even in high salinity media, was interpreted by π-π stacking hydrophobic interactions between the Phe residues, playing the role of “physical cross-linking”. These PICsomes were successfully loaded with siRNA directed against firefly luciferase enzyme expression. They also exhibit minimal cell cytotoxicity while superior silencing efficacy was shown by cell bioluminescence assay as compared to free siRNA and a standard lipofectamine-siRNA complex. As such, self-assembly of oppositely charged PEO-b-poly(amino acids) block copolymers enabled forming PICsomes of high stability thanks to π-π interactions of the Phe co-monomer in the polypeptide block, with high potential as biocompatible nanocarriers for RNA interference.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
INRAE
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Ingénierie des Systèmes Polymères

Submission date :

2023-10-10T08:39:17Z
2023-10-11T09:13:39Z