Title :

Stability of trace element concentrations in frozen urine - Effect on different elements of more than 10 years at -80°C

Author(s) :

Beauval, Nicolas [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Leroyer, Ariane [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Hisbergues, Michael [Auteur]
Allorge, Delphine [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Maboudou, Patrice [Auteur]
Howsam, Michael [Auteur]
Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167
Nisse, Catherine [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Journal title :

Journal of Trace Elements in Medicine and Biology

Abbreviated title :

J Trace Elem Med Biol

Volume number :

74

Pages :

127080

Publisher :

Elsevier

Publication date :

2022-10-02

ISSN :

0946-672X

English keyword(s) :

Trace elements
Stability
Freezing
Urine
Biobanking
ICP-MS

HAL domain(s) :

Sciences du Vivant [q-bio]
Chimie

English abstract : [en]

Background Analysis of urinary trace elements is widely used in Human Biology, especially in occupational and environmental biomonitoring. Collections of urine samples are of great interest for those studying trace ...
Show more >Background Analysis of urinary trace elements is widely used in Human Biology, especially in occupational and environmental biomonitoring. Collections of urine samples are of great interest for those studying trace elements but many of them are actually unused, in part perhaps because of a lack of knowledge about the stability of trace element concentrations under such storage conditions. The aim of this study was to evaluate the impact of a long-term frozen storage on the measurement of the urinary concentration of 10 trace elements. Method Forty-eight urinary samples were re-analysed by inductively coupled plasma mass spectrometry (ICP-MS) for the quantification of As, Cd, Co, Cr, Mn, Ni, Pb, Sb, Tl, and Zn, after 11–13 years of frozen storage at − 80 °C. Results A slight water loss likely occurred, even at − 80 °C, but seems to be compensated by using creatinine-adjusted concentrations. Concentrations of As, Cd, Pb, Tl, Zn, and possibly Mn, did not suffer from major modification during storage, while the plastic container likely contaminated samples with Sb. The technological evolution over 13 years may have affected some results, especially those with lower concentrations, and must be taken into account when comparing data over time. Conclusion This study provides some promising preliminary data on the stability of trace element concentrations during long-term frozen storage, and some evidence that urine samples in existing biobanks remain a valuable resource, even if they were collected many years ago.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille
Institut Pasteur de Lille

Collections :

• Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167
• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2023-10-20T06:00:43Z
2024-02-29T13:34:38Z