Title :

Exploring graphene-based materials' genotoxicity: inputs of a screening method

Author(s) :

Achawi, Salma [Auteur]
Huot, Ludovic [Auteur]
Institut Pasteur de Lille
Nesslany, Fabrice [Auteur]
Institut Pasteur de Lille
Pourchez, Jérémie [Auteur]
Simar, Sophie [Auteur]
Forest, Valérie [Auteur]
Feneon, Bruno [Auteur]

Journal title :

Nanotoxicology

Abbreviated title :

Nanotoxicology

Volume number :

15

Pages :

1279-1294

Publisher :

Taylor & Francis

Publication date :

2022-01-13

ISSN :

1743-5404

English keyword(s) :

Graphene-based materials
genotoxicity
high-throughput screening
structure-activity relationships

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Graphene-based materials (GBMs) are promising nanomaterials, and several innovations depend on their use. However, the assessment of their potential hazard must be carefully explored before entering any market. GBMs are ...
Show more >Graphene-based materials (GBMs) are promising nanomaterials, and several innovations depend on their use. However, the assessment of their potential hazard must be carefully explored before entering any market. GBMs are indeed well-known to induce various biological impacts, including oxidative stress, which can potentially lead to DNA damage. Genotoxicity is a major endpoint for hazard assessment and has been explored for GBMs, but the available literature shows conflicting results. In this study, we assessed the genotoxicity of 13 various GBMs, one carbon black and one amorphous silica through a DNA damage response assay (using a human respiratory cell model, BEAS-2B). Concurrently, oxidative stress was assessed through a ROS production quantification (DCFH-DA assay using a murine macrophage model, RAW 264.7). We also performed a full physicochemical characterization of our samples to explore potential structure-activity relationships involving genotoxicity. We observed that surface oxidation appears linked to genotoxicity response and were able to distinguish several groups within our studied GBMs showing different genotoxicity results. Our findings highlight the necessity to individually consider each nanoform of GBMs since the tested samples showed various results and modes of action. We propose this study as a genotoxicity assessment using a high-throughput screening method and suggest few hypotheses concerning the genotoxicity mode of action of GBMs.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Other project(s) or funding source(s) :

Michelin

Administrative institution(s) :

Université de Lille
CHU Lille
Institut Pasteur de Lille

Collections :

• Autres travaux scientifiques

Submission date :

2023-10-20T06:11:08Z
2024-02-29T11:29:04Z