Title :

Interleukin-1α Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation.

Author(s) :

Bernau, Ksenija [Auteur]
University of Wisconsin School of Medicine and Public Health
Leet, Jonathan P [Auteur]
University of Wisconsin School of Medicine and Public Health
Floerke, Heather [Auteur]
University of Wisconsin School of Medicine and Public Health
Bruhn, Ellen M [Auteur]
University of Wisconsin School of Medicine and Public Health
Noll, Andrea L [Auteur]
University of Wisconsin School of Medicine and Public Health
McDermott, Ivy S [Auteur]
University of Wisconsin School of Medicine and Public Health
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Jarjour, Nizar N [Auteur]
University of Wisconsin School of Medicine and Public Health
Sandbo, Nathan [Auteur]
University of Wisconsin School of Medicine and Public Health

Journal title :

Cells

Volume number :

10

Publication date :

2021-03-02

ISSN :

2073-4409

English keyword(s) :

Eosinophils
Fibroblasts
Humans
Inflammation
Interleukin-1alpha
IL1 receptor
IL1α
IL6
IL8
asthma
cytokines
eosinophil
fibroblast
intracellular signaling

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Eosinophils contribute to allergic inflammation in asthma in part via elaboration of a complex milieu of soluble mediators. Human bronchial fibroblasts (HBF) respond to stimulation by these mediators by acquiring a ...
Show more >Eosinophils contribute to allergic inflammation in asthma in part via elaboration of a complex milieu of soluble mediators. Human bronchial fibroblasts (HBF) respond to stimulation by these mediators by acquiring a pro-inflammatory profile including induction of interleukin 6 (IL6) and IL8. This study sought to determine key component(s) of eosinophil soluble factors that mediate IL6 and IL8 induction in HBF. HBF treated with eosinophil-derived soluble mediators were analyzed for gene expression, intracellular signaling, and IL6 and IL8 secretion following inhibition of inflammatory signaling. Segmental allergen bronchoprovocation (SBP-Ag) was performed in mild asthmatics and bronchoalveolar lavage fluid was analyzed for eosinophils and cytokines. We found that signaling via the IL1α/IL1 receptor is an essential component of the response of HBF to eosinophil-derived soluble factors. IL1α-dependent activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) signaling is required to induce IL6 secretion. However, NFκB signaling is dispensable for the induction of IL8, whereas Src is required. IL1α is associated with eosinophilic inflammation in human airways after SBP-Ag. Conclusions: IL1α appears to be a critical component of the soluble eosinophil-derived milieu that drives pro-inflammatory bronchial fibroblast responses and associates with eosinophilic inflammation following SBP-Ag. Disruption of IL1α-signaling could modify the downstream effects of eosinophilic inflammation on airway remodeling.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Autres travaux scientifiques

Submission date :

2023-10-21T11:27:51Z
2024-03-28T07:31:16Z