Observation and Quantification of Eosinophil ...
Document type :
Partie d'ouvrage: Chapitre
PMID :
Permalink :
Title :
Observation and Quantification of Eosinophil Motility
Author(s) :
Barretto, Karina T [Auteur]
Mosher, Deane F [Auteur]
Jarjour, Nizar N [Auteur]
Curran, Colleen S [Auteur]
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Johansson, Mats W [Auteur]
Mosher, Deane F [Auteur]
Jarjour, Nizar N [Auteur]
Curran, Colleen S [Auteur]
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Johansson, Mats W [Auteur]
Book title :
Eosinophils
Issue number :
Methods in Molecular Biology
Pages :
139-148
Publisher :
Springer Verlag
Publication date :
2021-06-24
ISSN :
1940-6029
English keyword(s) :
Allergens
Blood Proteins
Cell Migration Assays
Cell Movement
Cytoplasmic Granules
Eosinophil Granule Proteins
Eosinophils
Extracellular Matrix
Flow Cytometry
Humans
Microscopy
Crystalloid granule
Cytokines
Differential interference contrast video-enhanced microscopy
Extracellular matrix
Migration
Motility
Periostin
Blood Proteins
Cell Migration Assays
Cell Movement
Cytoplasmic Granules
Eosinophil Granule Proteins
Eosinophils
Extracellular Matrix
Flow Cytometry
Humans
Microscopy
Crystalloid granule
Cytokines
Differential interference contrast video-enhanced microscopy
Extracellular matrix
Migration
Motility
Periostin
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Eosinophils are important for tissue homeostasis and host responses to pathogens and allergens. The impact of eosinophils within tissues depends in part on whether cytotoxic proteins in crystalloid granules are released. ...
Show more >Eosinophils are important for tissue homeostasis and host responses to pathogens and allergens. The impact of eosinophils within tissues depends in part on whether cytotoxic proteins in crystalloid granules are released. Determinants of eosinophil motility and loss of granule contents are incompletely understood. The goal of this chapter is to present methods to study the effects of potential mediators on purified human blood eosinophils interacting with adhesive proteins found in extracellular matrix. We show that differential interference contrast video-enhanced microscopy and a bead-clearing assay provide complementary information about how different mediator-adhesive protein combinations direct eosinophil motility and granule fate. The former method is rich in information about cell shape, pattern of movement, and state of granules whereas the latter method lends itself to quantification and interrogation of multiple conditions in replicate.Show less >
Show more >Eosinophils are important for tissue homeostasis and host responses to pathogens and allergens. The impact of eosinophils within tissues depends in part on whether cytotoxic proteins in crystalloid granules are released. Determinants of eosinophil motility and loss of granule contents are incompletely understood. The goal of this chapter is to present methods to study the effects of potential mediators on purified human blood eosinophils interacting with adhesive proteins found in extracellular matrix. We show that differential interference contrast video-enhanced microscopy and a bead-clearing assay provide complementary information about how different mediator-adhesive protein combinations direct eosinophil motility and granule fate. The former method is rich in information about cell shape, pattern of movement, and state of granules whereas the latter method lends itself to quantification and interrogation of multiple conditions in replicate.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Submission date :
2023-10-21T11:30:13Z
2024-03-18T10:30:00Z
2024-03-18T10:30:00Z
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