Title :

Observation and Quantification of Eosinophil Motility

Author(s) :

Barretto, Karina T [Auteur]
Mosher, Deane F [Auteur]
Jarjour, Nizar N [Auteur]
Curran, Colleen S [Auteur]
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Johansson, Mats W [Auteur]

Book title :

Eosinophils

Issue number :

Methods in Molecular Biology

Pages :

139-148

Publisher :

Springer Verlag

Publication date :

2021-06-24

ISSN :

1940-6029

English keyword(s) :

Allergens
Blood Proteins
Cell Migration Assays
Cell Movement
Cytoplasmic Granules
Eosinophil Granule Proteins
Eosinophils
Extracellular Matrix
Flow Cytometry
Humans
Microscopy
Crystalloid granule
Cytokines
Differential interference contrast video-enhanced microscopy
Extracellular matrix
Migration
Motility
Periostin

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Eosinophils are important for tissue homeostasis and host responses to pathogens and allergens. The impact of eosinophils within tissues depends in part on whether cytotoxic proteins in crystalloid granules are released. ...
Show more >Eosinophils are important for tissue homeostasis and host responses to pathogens and allergens. The impact of eosinophils within tissues depends in part on whether cytotoxic proteins in crystalloid granules are released. Determinants of eosinophil motility and loss of granule contents are incompletely understood. The goal of this chapter is to present methods to study the effects of potential mediators on purified human blood eosinophils interacting with adhesive proteins found in extracellular matrix. We show that differential interference contrast video-enhanced microscopy and a bead-clearing assay provide complementary information about how different mediator-adhesive protein combinations direct eosinophil motility and granule fate. The former method is rich in information about cell shape, pattern of movement, and state of granules whereas the latter method lends itself to quantification and interrogation of multiple conditions in replicate.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Autres travaux scientifiques

Submission date :

2023-10-21T11:30:13Z
2024-03-18T10:30:00Z