Title :

Two-phase kinetics of biofilm removal during CIP. Respective roles of mechanical and chemical effects on the detachment of single cells vs cell clusters from a Pseudomonas fluorescens biofilm

Author(s) :

Benezech, Thierry [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]

Journal title :

Journal of Food Engineering

Volume number :

219

Pages :

121-128

Publication date :

2018-02

HAL domain(s) :

Sciences du Vivant [q-bio]/Ingénierie des aliments
Physique [physics]/Matière Condensée [cond-mat]/Science des matériaux [cond-mat.mtrl-sci]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
Sciences de l'ingénieur [physics]/Génie des procédés

English abstract : [en]

The objective of this study was to investigate the roles of mechanical and chemical actions on the detachment kinetics during CIP of biofilms of Pseudomonas fluorescens at a pilot-plant scale. Biofilms were grown 48 h at ...
Show more >The objective of this study was to investigate the roles of mechanical and chemical actions on the detachment kinetics during CIP of biofilms of Pseudomonas fluorescens at a pilot-plant scale. Biofilms were grown 48 h at 20 C in diluted milk. Shear stress conditions ranged from 0 (static) to 20 Pa and NaOH concentrations from 0.1 to 0.5% w/w. A simple two-phase model was applied to model the removal kinetics and compare detachment parameters. A quick removal of the biofilm during the first phase (4e5 log decrease in three minutes) was followed by a second phase of slow removal of the cells and small aggregates still present at the surface (0e2 log decrease). Hydrodynamics was responsible for removal of both biofilm and single cells while chemicals mainly disrupted biofilm clusters during the first phase. No complete biofilm removal was observed, suggesting a significant role of the interaction forces between bacteria and substrata in the CIP efficiency.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
ENSCL
CNRS
INRA

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2019-05-16T17:21:39Z
2021-05-17T14:18:02Z
2023-12-21T09:28:11Z