Fetal acidosis in a sheep model: can we ...
Document type :
Article dans une revue scientifique: Article original
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Title :
Fetal acidosis in a sheep model: can we perform a second day of protocol in order to reduce the numbers of animals needed?
Author(s) :
Pierre, Clementine [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Ghesquiere, Louise [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
De Jonckheere, Julien [Auteur]
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Aubry, Estelle [Auteur]
Environnement Périnatal et Santé - EA 4489
Sharma, Dyuti [Auteur]
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Deruelle, Phillipe [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Storme, Laurent [Auteur]
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Debarge, Veronique [Auteur]
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Garabedian, Charles [Auteur]
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Environnement périnatal et croissance - EA 4489 [EPS]
Ghesquiere, Louise [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
De Jonckheere, Julien [Auteur]
![refId](/themes/Mirage2//images/idref.png)
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Aubry, Estelle [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Environnement Périnatal et Santé - EA 4489
Sharma, Dyuti [Auteur]
![refId](/themes/Mirage2//images/idref.png)
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Deruelle, Phillipe [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Storme, Laurent [Auteur]
![refId](/themes/Mirage2//images/idref.png)
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Debarge, Veronique [Auteur]
![refId](/themes/Mirage2//images/idref.png)
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Garabedian, Charles [Auteur]
![refId](/themes/Mirage2//images/idref.png)
METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694
Journal title :
Clinical and experimental obstetrics & gynecology
Abbreviated title :
Clin. Exp. Obstet. Gynecol.
Volume number :
47
Pages :
79-83
Publication date :
2020-03-14
ISSN :
0390-6663
English keyword(s) :
Fetal sheep
Animal experimentation
Animal ethics
3R
Umbilical cord occlusion
Fetal acidosis
Animal experimentation
Animal ethics
3R
Umbilical cord occlusion
Fetal acidosis
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min ...
Show more >Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min of total umbilical cord occlusion (UCO) every 2.5 min was performed on fetal sheep (n=7) until pH reached 7.10. Chronic instrumentation regularly recorded arterial blood gas and hemodynamic parameters. The occlusions were repeated to compare the data between the 2 days for each animal. Results: At the end of the UCO period, fetal acidosis was obtained with a pH 7.14 on day 1 (D1) and 7.06 on day 2 (D2). At baseline, fetuses were more hypotensive at D2 (41 mmHg vs 44 mmHg on D1, p < 0.05). During the UCO period, there was no significant difference in blood gas and hemodynamic data between D1 and D2. Conclusion: To reduce the number of animals needed, performing a second experiment on the same animal appears possible even if results must be interpreted cautiously when severe acidosis is reached.Show less >
Show more >Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min of total umbilical cord occlusion (UCO) every 2.5 min was performed on fetal sheep (n=7) until pH reached 7.10. Chronic instrumentation regularly recorded arterial blood gas and hemodynamic parameters. The occlusions were repeated to compare the data between the 2 days for each animal. Results: At the end of the UCO period, fetal acidosis was obtained with a pH 7.14 on day 1 (D1) and 7.06 on day 2 (D2). At baseline, fetuses were more hypotensive at D2 (41 mmHg vs 44 mmHg on D1, p < 0.05). During the UCO period, there was no significant difference in blood gas and hemodynamic data between D1 and D2. Conclusion: To reduce the number of animals needed, performing a second experiment on the same animal appears possible even if results must be interpreted cautiously when severe acidosis is reached.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CHU Lille
CHU Lille
Collections :
Submission date :
2023-11-15T09:39:52Z
2023-12-14T11:01:21Z
2023-12-14T11:01:21Z
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